MicroRNA-194 Inhibits the Epithelial-Mesenchymal Transition in Gastric Cancer Cells by Targeting FoxM1

被引：56

作者：

Li, Zhenjun ^{[1
]}

Ying, Xiaojiang ^{[1
]}

Chen, Hongliang ^{[1
]}

Ye, Pingjiang ^{[1
]}

Shen, Yi ^{[1
]}

Pan, Weihuo ^{[1
]}

Zhang, Lihua ^{[1
]}

机构：

[1] Zhejiang Univ, Shaoxing Hosp, Shaoxing Peoples Hosp, Dept Colorectal Surg, Shaoxing 312000, Zhejiang, Peoples R China

来源：

DIGESTIVE DISEASES AND SCIENCES | 2014年 / 59卷 / 09期

关键词：

FoxM1; Epithelial-to-mesenchymal transition; miR-194; TRANSCRIPTION FACTOR FOXM1; EXPRESSION; MIR-194; ANGIOGENESIS; PROGRESSION; MICE;

D O I：

10.1007/s10620-014-3159-6

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We hypothesized that miR-194 may control Forkhead box protein M1 (FoxM1) expression in gastric cancer cells and therefore may have therapeutic potential in gastric cancer. The expression level of miR-194 was examined using real-time PCR in human gastric cancer and noncancerous gastric tissues, gastric cancer cell and normal gastric mucosal epithelial cell. We examined whether the miR-194 regulates cell migration and invasion, and the epithelial-mesenchymal transition Phenotype by inhibiting FoxM1 in gastric cancer cells. The expression of miR-194 was significantly lower in gastric cancer compared with non-cancerous gastric tissues and cells. Exogenous expression of miR-194 inhibited cell migration, invasion, and the epithelial-mesenchymal transition phenotype in gastric cancer cells. Moreover, we discovered a novel post-transcriptional regulatory mechanism of FoxM1 expression that is mediated by miR-194. Our study clearly demonstrates that miR-194 inhibits the acquisition of the EMT phenotype in gastric cancer cells by downregulating FoxM1, thereby inhibiting cell migration and invasion during cancer progression.

引用

页码：2145 / 2152

页数：8

共 22 条

[11] Modulation of MicroRNA-194 and Cell Migration by HER2-Targeting Trastuzumab in Breast Cancer [J].

Le, Xiao-Feng ;

Almeida, Maria I. ;

Mao, Weiqun ;

Spizzo, Riccardo ;

Rossi, Simona ;

Nicoloso, Milena S. ;

Zhang, Shu ;

Wu, Yun ;

Calin, George A. ;

Bast, Robert C., Jr. .

PLOS ONE, 2012, 7 (07)

[12] Critical Role and Regulation of Transcription Factor FoxM1 in Human Gastric Cancer Angiogenesis and Progression [J].

Li, Qiang ;

Zhang, Nu ;

Jia, Zhiliang ;

Le, Xiangdong ;

Dai, Bingbing ;

Wei, Daoyan ;

Huang, Suyun ;

Tan, Dongfeng ;

Xie, Keping .

CANCER RESEARCH, 2009, 69 (08) :3501-3509

[13] Analysis of Gene Expression Regulated by the ETV5 Transcription Factor in OV90 Ovarian Cancer Cells Identifies FOXM1 Overexpression in Ovarian Cancer [J].

Llaurado, Marta ;

Majem, Blanca ;

Castellvi, Josep ;

Cabrera, Silvia ;

Gil-Moreno, Antonio ;

Reventos, Jaume ;

Ruiz, Anna .

MOLECULAR CANCER RESEARCH, 2012, 10 (07) :914-924

[14] MicroRNAs: something important between the genes [J].

Mallory, AC ;

Vaucheret, H .

CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (02) :120-125

[15] miR-194 Is a Marker of Hepatic Epithelial Cells and Suppresses Metastasis of Liver Cancer Cells in Mice [J].

Meng, Zhipeng ;

Fu, Xianghui ;

Chen, Xiaosong ;

Zeng, Samuel ;

Tian, Yan ;

Jove, Richard ;

Xu, Rongzhen ;

Huang, Wendong .

HEPATOLOGY, 2010, 52 (06) :2148-2157

[16]

Mosavi-Jarrahi Alireza, 2006, Asian Pac J Cancer Prev, V7, P375

[17] Inverse Association between miR-194 Expression and Tumor Invasion in Gastric Cancer [J].

Song, Yongxi ;

Zhao, Feng ;

Wang, Zhenning ;

Liu, Zhuangkai ;

Chiang, Yeunpo ;

Xu, Yingying ;

Gao, Peng ;

Xu, Huimian .

ANNALS OF SURGICAL ONCOLOGY, 2012, 19 :S509-S517

[18] p53-Responsive miR-194 Inhibits Thrombospondin-1 and Promotes Angiogenesis in Colon Cancers [J].

Sundaram, Prema ;

Hultine, Stacy ;

Smith, Lauren M. ;

Dews, Michael ;

Fox, Jamie L. ;

Biyashev, Dauren ;

Schelter, Janell M. ;

Huang, Qihong ;

Cleary, Michele A. ;

Volpert, Olga V. ;

Thomas-Tikhonenko, Andrei .

CANCER RESEARCH, 2011, 71 (24) :7490-7501

[19] Forkhead box M1 transcription factor: A novel target for cancer therapy [J].

Wang, Zhiwei ;

Ahmad, Aamir ;

Li, Yiwei ;

Banerjee, Sanjeev ;

Kong, Dejuan ;

Sarkar, Fazlul H. .

CANCER TREATMENT REVIEWS, 2010, 36 (02) :151-156

[20] MicroRNA profiling of human gastric cancer [J].

Yao, Yu ;

Suo, Ai-Li ;

Li, Zong-Fang ;

Liu, Li-Ying ;

Tian, Tao ;

Ni, Lei ;

Zhang, Wang-Gang ;

Nan, Ke-Jun ;

Song, Tu-Sheng ;

Huang, Chen .

MOLECULAR MEDICINE REPORTS, 2009, 2 (06) :963-970

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