MicroRNA-194 Inhibits the Epithelial-Mesenchymal Transition in Gastric Cancer Cells by Targeting FoxM1

被引：56
作者：
Li, Zhenjun [1 ]
Ying, Xiaojiang [1 ]
Chen, Hongliang [1 ]
Ye, Pingjiang [1 ]
Shen, Yi [1 ]
Pan, Weihuo [1 ]
Zhang, Lihua [1 ]
机构：
[1] Zhejiang Univ, Shaoxing Hosp, Shaoxing Peoples Hosp, Dept Colorectal Surg, Shaoxing 312000, Zhejiang, Peoples R China
来源：
DIGESTIVE DISEASES AND SCIENCES | 2014年 / 59卷 / 09期
关键词：
FoxM1; Epithelial-to-mesenchymal transition; miR-194; TRANSCRIPTION FACTOR FOXM1; EXPRESSION; MIR-194; ANGIOGENESIS; PROGRESSION; MICE;
D O I：
10.1007/s10620-014-3159-6
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
We hypothesized that miR-194 may control Forkhead box protein M1 (FoxM1) expression in gastric cancer cells and therefore may have therapeutic potential in gastric cancer. The expression level of miR-194 was examined using real-time PCR in human gastric cancer and noncancerous gastric tissues, gastric cancer cell and normal gastric mucosal epithelial cell. We examined whether the miR-194 regulates cell migration and invasion, and the epithelial-mesenchymal transition Phenotype by inhibiting FoxM1 in gastric cancer cells. The expression of miR-194 was significantly lower in gastric cancer compared with non-cancerous gastric tissues and cells. Exogenous expression of miR-194 inhibited cell migration, invasion, and the epithelial-mesenchymal transition phenotype in gastric cancer cells. Moreover, we discovered a novel post-transcriptional regulatory mechanism of FoxM1 expression that is mediated by miR-194. Our study clearly demonstrates that miR-194 inhibits the acquisition of the EMT phenotype in gastric cancer cells by downregulating FoxM1, thereby inhibiting cell migration and invasion during cancer progression.
引用
收藏
页码：2145 / 2152
页数：8
相关论文
共 22 条
[1]   RETRACTED: Over-Expression of FoxM1 Leads to Epithelial-Mesenchymal Transition and Cancer Stem Cell Phenotype in Pancreatic Cancer Cells (Retracted article. See vol. 117, pg. 1963, 2016) [J].
Bao, Bin ;
Wang, Zhiwei ;
Ali, Shadan ;
Kong, Dejuan ;
Banerjee, Sanjeev ;
Ahmad, Aamir ;
Li, Yiwei ;
Azmi, Asfar S. ;
Miele, Lucio ;
Sarkar, Fazlul H. .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2011, 112 (09) :2296-2306
[2]   Tight correlation between expression of the Forkhead transcription factor FOXM1 and HER2 in human breast cancer [J].
Bektas, Nuran ;
ten Haaf, Anette ;
Veeck, Juergen ;
Wild, Peter Johannes ;
Luescher-Firzlaff, Juliane ;
Hartmann, Arndt ;
Knuechel, Ruth ;
Dahl, Edgar .
BMC CANCER, 2008, 8 (1)
[3]   p53-Responsive MicroRNAs 192 and 215 Are Capable of Inducing Cell Cycle Arrest [J].
Braun, Christian J. ;
Zhang, Xin ;
Savelyeva, Irina ;
Wolff, Sonja ;
Moll, Ute M. ;
Schepeler, Troels ;
Orntoft, Torben F. ;
Andersen, Claus L. ;
Dobbelstein, Matthias .
CANCER RESEARCH, 2008, 68 (24) :10094-10104
[4]   MicroRNA signatures in human cancers [J].
Calin, George A. ;
Croce, Carlo M. .
NATURE REVIEWS CANCER, 2006, 6 (11) :857-866
[5]   MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1 [J].
Dong, Peixin ;
Kaneuchi, Masanori ;
Watari, Hidemichi ;
Hamada, Junichi ;
Sudo, Satoko ;
Ju, Jingfang ;
Sakuragi, Noriaki .
MOLECULAR CANCER, 2011, 10
[6]   FoxM1 is Overexpressed in Helicobacter pylori-Induced Gastric Carcinogenesis and Is Negatively Regulated by miR-370 [J].
Feng, Yimin ;
Wang, Lixiang ;
Zeng, Jiping ;
Shen, Li ;
Liang, Xiuming ;
Yu, Han ;
Liu, Shili ;
Liu, Zhifang ;
Sun, Yundong ;
Li, Wenjuan ;
Chen, Chunyan ;
Jia, Jihui .
MOLECULAR CANCER RESEARCH, 2013, 11 (08) :834-844
[7]   Coordinated Regulation of Cell Cycle Transcripts by p53-inducible microRNAs, miR-192 and miR-215 [J].
Georges, Sara A. ;
Biery, Matthew C. ;
Kim, Soo-yeon ;
Schelter, Janell M. ;
Guo, Jane ;
Chang, Aaron N. ;
Jackson, Aimee L. ;
Carleton, Michael O. ;
Linsley, Peter S. ;
Cleary, Michele A. ;
Chau, B. Nelson .
CANCER RESEARCH, 2008, 68 (24) :10105-10112
[8]   Increased levels of the FoxM1 transcription factor accelerate development and progression of prostate carcinomas in both TRAMP and LADY transgenic mice [J].
Kalin, TV ;
Wang, IC ;
Ackerson, TJ ;
Major, ML ;
Detrisac, CJ ;
Kalinichenko, VV ;
Lyubimov, A ;
Costa, RH .
CANCER RESEARCH, 2006, 66 (03) :1712-1720
[9]   FoxM1 is required for execution of the mitotic programme and chromosome stability [J].
Laoukili, J ;
Kooistra, MRH ;
Brás, A ;
Kauw, J ;
Kerkhoven, RM ;
Morrison, A ;
Clevers, H ;
Medema, RH .
NATURE CELL BIOLOGY, 2005, 7 (02) :126-U34
[10]   FoxM1: At the crossroads of ageing and cancer [J].
Laoukili, Jamila ;
Stahl, Marie ;
Medema, Rene H. .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2007, 1775 (01) :92-102
123