Modulation of genes related to the recruitment of immune cells in the digestive tract of trout experimentally infected with infectious pancreatic necrosis virus (IPNV) or orally vaccinated

被引:34
作者
Ballesteros, Natalia A. [1 ]
Rodriguez Saint-Jean, Sylvia [1 ]
Perez-Prieto, Sara I. [1 ]
Aquilino, Carolina [2 ]
Tafalla, Carolina [2 ]
机构
[1] CSIC, Ctr Invest Biol, Dpto Microbiol Mol & Biol Infecc, Madrid 28040, Spain
[2] INIA, CISA, Madrid 28130, Spain
基金
欧盟第七框架计划;
关键词
Infectious pancreatic necrosis virus (IPNV); DNA vaccine; Rainbow trout; Chemokines; Digestive tract; Leukocyte recruitment; RAINBOW-TROUT; DIFFERENTIAL EXPRESSION; INTESTINAL EPITHELIUM; T-CELLS; LYMPHOCYTES; MIGRATION; MUCOSAL; CCR7; IGT;
D O I
10.1016/j.dci.2013.12.009
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
There are still many details of how intestinal immunity is regulated that remain unsolved in teleost. Although leukocytes are present all along the digestive tract, most immunological studies have focused on the posterior segments and the importance of each gut segment in terms of immunity has barely been addressed. In the current work, we have studied the regulation of several immune genes along five segments of the rainbow trout (Oncorhynchus mykiss) digestive tract, comparing the effects observed in response to an infectious pancreatic necrosis virus (IPNV) infection to those elicited by oral vaccination with a plasmid coding for viral VP2. We have focused on the regulation of several mucosal chemokines, chemoldne receptors, the major histocompatibility complex II (MHC-II) and tumor necrosis factor a (TNFa). Furthermore, the recruitment of IOC cells and CD3 cells was evaluated along the different segments in response to IPNV by immunohistochemical techniques. Our results provide evidences that there is a differential regulation of these immune genes in response to both stimuli along the gut segments. Along with this chemokine and chemokine receptor induction, IPNV provoked a mobilization of IgM and Igr cells to the foregut and pyloric caeca region, and CD3 cells to the pyloric caeca and midgut/hindgut regions. Our results will contribute to a better understanding of how mucosal immunity is orchestrated in the different gut segments of teleost (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:195 / 205
页数:11
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