共 37 条
Pup, a prokaryotic ubiquitin-like protein, is an intrinsically disordered protein
被引:78
作者:
Liao, Shanhui
[1
]
Shang, Qiang
[1
]
Zhang, Xuecheng
[2
]
Zhang, Jiahai
[1
]
Xu, Chao
[1
]
Tu, Xiaoming
[1
]
机构:
[1] Univ Sci & Technol China, Sch Life Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China
[2] Anhui Univ, Sch Life Sci, Hefei 230039, Anhui, Peoples R China
关键词:
Intrinsically disordered protein;
NMR spectroscopy;
prokaryote;
protein degradation;
Pup;
ubiquitin;
CHEMICAL-SHIFTS;
PROTEASOME;
D O I:
10.1042/BJ20090738
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Pup (prokaryotic ubiquitin-like protein) from Mycobacterium tuberculosis is the first ubiquitin-like protein identified in non-eukaryotic cells. Although different ubiquitin-like proteins from eukaryotes share low sequence similarity, their 3D (three-dimensional) structures exhibit highly conserved typical ubiquitin-like folds. Interestingly, our studies reveal that Pup not only shares low sequence similarity, but also presents a totally distinguished structure compared with other ubiquitin-like superfamily proteins. Diverse structure predictions combined with CD and NMR spectroscopic studies all demonstrate that Pup is an intrinsically disordered protein. Moreover, H-1-N-15 NOE (nuclear Overhauser effect) data and CSI (chemical shift index) analyses indicate that there is a residual secondary structure at the C-terminus of Pup. In M. tuberculosis, Mpa (mycobacterium proteasomal ATPase) is the regulatory cap ATPase of the proteasome that interacts with Pup and brings the substrates to the proteasome for degradation. In the present paper, SPR (Surface plasmon resonance) and NMR perturbation studies imply that the C-terminus of Pup, ranging from residues 30 to 59, binds to Mpa probably through a hydrophobic interface. In addition, phylogenetic analysis clearly shows that the Pup family belongs to a Unique and divergent evolutionary branch, Suggesting that it is the most ancient and deeply branched family among ubiquitin-like proteins. This might explain the structural distinction between Pup and other ubiquitin-like superfamily proteins.
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页码:207 / 215
页数:9
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