Genetic relationships among six Iranian indigenous sheep populations based on microsatellite analysis

被引:11
作者
Molaee, V. [1 ]
Osfoori, R. [2 ]
Nasab, M. P. Eskandari [1 ]
Qanbari, S. [1 ]
机构
[1] Univ Zanjan, Fac Agr, Dept Anim Sci, Zanjan, Iran
[2] ABRII, Karaj, Iran
关键词
Genetic relationship; Iranian indigenous population; Microsatellite; Sheep; DISTANCE; BREEDS;
D O I
10.1016/j.smallrumres.2009.05.004
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Six Iranian indigenous sheep populations: Ghashghaee (GSH), Bakhtiyari (BKH), Kabude Shiraz (KSH), Sanjabi (SAN), Lori (LRI) and Arabi Khuzestan (ARB) were genotyped for 10 microsatellite markers recommended by the MoDAD program IFAO, 1998. Food and Agriculture Organisation of the United Nations Secondary Guidelines for Development of National Farm Animal Genetic Resources Management Plans. Measurement of Domestic Animal Diversity (MoDAD). Recommended Microsatellite Markers]. All population per locus combinations were at Hardy-Weinberg Equilibrium except MAF214 for KSH and BM1824, MAF214 and MCM527 for SAN (P < 0.05). All loci were polymorphic in all populations. There was substantial genetic variability within sheep populations, with average heterozygosity range of 0.747-0.792 based on expected hetrozygosity. The three sheep populations showing the highest level of inbreeding on the basis of heterozygote deficiency. The lowest genetic distance (0.166) was obtained between LRI and BKH and the highest genetic distance (0.378) between SAN and GSH. The phylogenetic tree based on unbiased distances was drawn using UPGMA. To study the genetic relationships among sheep populations, a principal coordinate analysis (PCA) based on Nei standard distances was performed. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:121 / 124
页数:4
相关论文
共 20 条
[1]  
[Anonymous], 1978, EVOLUTION GENETIC PO
[2]  
CRAWFORD AM, 1995, GENETICS, V140, P703
[3]  
*FAO, 2000, DOM AN DIV INF SYST
[4]  
GRIGALINAIT MT, 2003, MICROSATELITTE VARIA, V22, P69
[5]  
Gustavo A., 2000, CONSERV GENET, V1, P3
[6]  
HANDLEY LJL, 2007, GENETIC STRUCTURE EU, P1
[7]  
LAWSON R, 1989, AM ZOOL, V29, P863
[8]   A SIMPLE SALTING OUT PROCEDURE FOR EXTRACTING DNA FROM HUMAN NUCLEATED CELLS [J].
MILLER, SA ;
DYKES, DD ;
POLESKY, HF .
NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :1215-1215
[9]   GENETIC DISTANCE BETWEEN POPULATIONS [J].
NEI, M .
AMERICAN NATURALIST, 1972, 106 (949) :283-+
[10]  
NEI M, 1978, GENETICS, V89, P583