The whey acidic protein family: A new signature motif and three-dimensional structure by comparative modeling

被引：128
作者：
Ranganathan, S
Simpson, KJ
Shaw, DC
Nicholas, KR
机构：
[1] Univ Sydney, Australian Genom Informat Ctr, Sydney, NSW 2006, Australia
[2] Victorian Inst Anim Sci, Attwood, Vic, Australia
[3] La Trobe Univ, Sch Agr Sci, Bundoora, Vic 3083, Australia
[4] Australian Natl Univ, John Curtin Sch Med Res, Prot Biochem Grp, Canberra, ACT 2601, Australia
来源：
JOURNAL OF MOLECULAR GRAPHICS & MODELLING | 1999年 / 17卷 / 02期
基金：
澳大利亚研究理事会;
关键词：
WAP; molecular model; sequence motifs; sequence-structure comparison; molecular electrostatic potential; four-disulfide core;
D O I：
10.1016/S1093-3263(99)00023-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Whey acidic proteins (WAP) from the mouse, rat, rabbit, camel, and pig comprise two "four-disulfide core" domains. From a detailed analysis of all sequences containing this domain, we propose a new PROSITE motif ([KRHGVLN]-X-{PF}-X-[CF]-[PQSVLI]-X(9, 19)-C-{P}-X-[DN]-X-{N}-[CE]-X(5)-C-C) to accurately model for the WAP proteins is proposed, bread on the human mucous proteinase inhibitor crystal structure. This article presents a detailed atomic model for the two-domain porcine WAP sequence by comparitive modeling. Surface electrostatic potential calculation indicate that the second domain of the pig WAP model is similar to the functional human mucous proteinase inhibitor domains, whereas the first domain may be nonfunctional. (C) 2000 by Elsevier Science Inc.
引用
收藏
页码：106 / +
页数：11
相关论文
共 34 条
  • [1] BARANYI N, 1995, COMP BIOCH PHYSL B, V113, P407
  • [2] Pfam 3.1: 1313 multiple alignments and profile HMMs match the majority of proteins
    Bateman, A
    Birney, E
    Durbin, R
    Eddy, SR
    Finn, RD
    Sonnhammer, ELL
    [J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (01) : 260 - 262
  • [3] BEG OU, 1986, EUR J BIOCHEM, V159, P195, DOI 10.1111/j.1432-1033.1986.tb09852.x
  • [4] COMPARISON OF THE WHEY ACIDIC PROTEIN GENES OF THE RAT AND MOUSE
    CAMPBELL, SM
    ROSEN, JM
    HENNIGHAUSEN, LG
    STRECHJURK, U
    SIPPEL, AE
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (22) : 8685 - 8697
  • [5] Recent improvements of the ProDom database of protein domain families
    Corpet, F
    Gouzy, J
    Kahn, D
    [J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (01) : 263 - 267
  • [6] SELECTIVE-INHIBITION OF MICROBIAL SERINE PROTEASES BY ENAP-2, AN ANTIMICROBIAL PEPTIDE FROM EQUINE NEUTROPHILS
    COUTO, MA
    HARWIG, SSL
    LEHRER, RI
    [J]. INFECTION AND IMMUNITY, 1993, 61 (07) : 2991 - 2994
  • [7] THE WDNM1 GENE-PRODUCT IS A NOVEL MEMBER OF THE 4-DISULFIDE CORE FAMILY OF PROTEINS
    DEAR, TN
    KEFFORD, RF
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (01) : 247 - 254
  • [8] RECENT DATA ON THE STRUCTURE OF RABBIT MILK PROTEIN GENES AND ON THE MECHANISM OF THE HORMONAL-CONTROL OF THEIR EXPRESSION
    DEVINOY, E
    HUBERT, C
    JOLIVET, G
    THEPOT, D
    CLERGUE, N
    DESALEUX, M
    DION, M
    SERVELY, JL
    HOUDEBINE, LM
    [J]. REPRODUCTION NUTRITION DEVELOPMENT, 1988, 28 (4B): : 1145 - 1164
  • [9] Solution structure of r-elafin, a specific inhibitor of elastase
    Francart, C
    Dauchez, M
    Alix, AJP
    Lippens, G
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 268 (03) : 666 - 677
  • [10] Molecular characterization of a ribonucleic acid transcript that is highly up-regulated at the time of ovulation in the brook trout (Salvelinus fontinalis) ovary
    Garczynski, MA
    Goetz, FW
    [J]. BIOLOGY OF REPRODUCTION, 1997, 57 (04) : 856 - 864
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