A Biochemical Characterization of the DNA Binding Activity of the Response Regulator VicR from Streptococcus mutans

被引：22

作者：

Ayala, Eduardo ^{[1
]}

Downey, Jennifer S. ^{[1
]}

Mashburn-Warren, Lauren ^{[2
]}

Senadheera, Dilani B. ^{[3
]}

Cvitkovitch, Dennis G. ^{[3
]}

Goodman, Steven D. ^{[1
,2
]}

机构：

[1] Univ So Calif, Dept Mol & Computat Biol, Div Biomed Sci, Herman Ostrow Sch Dent, Los Angeles, CA 90045 USA

[2] Nationwide Childrens Hosp, Res Inst, Ctr Microbial Pathogenesis, Columbus, OH USA

[3] Univ Toronto, Fac Dent, Dent Res Inst, Toronto, ON, Canada

来源：

PLOS ONE | 2014年 / 9卷 / 09期

关键词：

YYCG/YYCF 2-COMPONENT SYSTEM; BIOFILM FORMATION; GENETIC COMPETENCE; SEQUENCE-ANALYSIS; PROTEIN; PHOSPHORYLATION; TRANSCRIPTION; EXPRESSION; PROMOTER; IDENTIFICATION;

D O I：

10.1371/journal.pone.0108027

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Two-component systems (TCSs) are ubiquitous among bacteria and are among the most elegant and effective sensing systems in nature. They allow for efficient adaptive responses to rapidly changing environmental conditions. In this study, we investigated the biochemical characteristics of the Streptococcus mutans protein VicR, an essential response regulator that is part of the VicRK TCS. We dissected the DNA binding requirements of the recognition sequences for VicR in its phosphorylated and unphosphorylated forms. In doing so, we were able to make predictions for the expansion of the VicR regulon within S. mutans. With the ever increasing number of bacteria that are rapidly becoming resistant to even the antibiotics of last resort, TCSs such as the VicRK provide promising targets for a new class of antimicrobials.

引用

页数：11

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