An improved large-scale screening method for assessment of Colletotrichum coccodes aggressiveness using mature green tomatoes

被引：8
作者：
Ben-Daniel, B. [1 ,2 ]
Bar-Zvi, D. [2 ]
Tsror , L. [1 ]
机构：
[1] Agr Res Org, Dept Plant Pathol, Gilat Res Ctr, IL-85280 M P Negev, Israel
[2] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
来源：
PLANT PATHOLOGY | 2009年 / 58卷 / 03期
关键词：
anthracnose; black dot; Solanum lycopersicum; Solanum tuberosum; VEGETATIVE COMPATIBILITY GROUPS; BLACK DOT; CAUSAL AGENT; DISEASE; DURATION; INOCULUM;
D O I：
10.1111/j.1365-3059.2008.01989.x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A reliable, rapid and low-cost screening bioassay, based on measuring lesion sizes, rotten tissue weight and tissue colonization 1 week after the injection of a conidial suspension into the stem scars of mature green tomato fruits, was developed to assess the aggressiveness of Colletotrichum coccodes isolates. This protocol was compared with inoculation of either potato plantlets from in vitro multiplication, or ripe tomato fruits, using C. coccodes isolates from potato and from tomato. Aggressiveness to mature green tomato fruits was scored by measuring lesion size and weight at the scar end of the fruit, while colonization was measured in samples taken from the blossom end. Values of all three disease parameters were significantly higher (ca. 3 times) with aggressive isolates of the pathogen than with less aggressive ones. High correlation levels among these three parameters were obtained. Also, the aggressiveness to mature green tomatoes was highly correlated with the aggressiveness to potato tissue culture plantlets. It was concluded that lesion size in inoculated tomatoes can be used as a sole measure for estimating the aggressiveness of C. coccodes isolates to either tomato or potato.
引用
收藏
页码：497 / 503
页数：7
相关论文
共 15 条
[1]  
BARKSDALE TH, 1969, PHYTOPATHOLOGY, V59, P1373
[2]   CHANGES IN GENE-EXPRESSION DURING TOMATO FRUIT RIPENING [J].
BIGGS, MS ;
HARRIMAN, RW ;
HANDA, AK .
PLANT PHYSIOLOGY, 1986, 81 (02) :395-403
[3]   Distribution and fungicide sensitivity of fungal pathogens causing anthracnose-like lesions on tomatoes grown in Ohio [J].
Chapin, LJG ;
Wang, Y ;
Lutton, E ;
Gardener, BBM .
PLANT DISEASE, 2006, 90 (04) :397-403
[4]   EFFECT OF TEMPERATURE, WETNESS DURATION, AND INOCULUM DENSITY ON INFECTION AND LESION DEVELOPMENT OF COLLETOTRICHUM-COCCODES ON TOMATO FRUIT [J].
DILLARD, HR .
PHYTOPATHOLOGY, 1989, 79 (10) :1063-1066
[5]   Disease progress of black dot on tomato roots and reduction in incidence with foliar applied fungicides [J].
Dillard, HR ;
Cobb, AC .
PLANT DISEASE, 1997, 81 (12) :1439-1442
[6]  
DILLARD HR, 1992, BIOL PATHOLOGY CONTR, P225
[7]   Black dot (Colletotrichum coccodes):: an increasingly important disease of potato [J].
Lees, AK ;
Hilton, AJ .
PLANT PATHOLOGY, 2003, 52 (01) :3-12
[8]   Vegetative compatibility groups and aggressiveness of North American isolates of Colletotrichum coccodes, the causal agent of potato black dot [J].
Nitzan, N. ;
Tsror, L. ;
Johnson, D. A. .
PLANT DISEASE, 2006, 90 (10) :1287-1292
[9]   Effect of seed-tuber generation, soilborne inoculum, and azoxystrobin application on development of potato black dot caused by Colletotrichum coccodes [J].
Nitzan, N ;
Cummings, TF ;
Johnson, DA .
PLANT DISEASE, 2005, 89 (11) :1181-1185
[10]   Vegetative compatibility groups in Colletotrichum coccodes, the causal agent of black dot on potato [J].
Nitzan, N ;
Hazanovsky, M ;
Tal, M ;
Tsror, L .
PHYTOPATHOLOGY, 2002, 92 (08) :827-832
12