Iron overload and gene expression in HepG2 cells: analysis by differential display

被引：22

作者：

Barisani, D

Meneveri, R

Ginelli, E

Cassani, C

Conte, D

机构：

[1] Osped Maggiore, IRCCS, Cattedra Gastroenterol, I-20122 Milan, Italy

[2] Univ Milan, Dipartimento Biol & Genet Sci Med, I-20133 Milan, Italy

来源：

FEBS LETTERS | 2000年 / 469卷 / 2-3期

关键词：

differential display; hepatocyte; antioxidant; apolipoprotein B; semaphorin cd100; aldose reductase;

D O I：

10.1016/S0014-5793(00)01280-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study,vas to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects sere time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways. (C) 2000 Federation of European Biochemical Societies.

引用

页码：208 / 212

页数：5

共 34 条

[21] ORGANIZATION OF THE HUMAN TRANSFERRIN GENE - DIRECT EVIDENCE THAT IT ORIGINATED BY GENE DUPLICATION [J].