RNAi mediated inhibition of viroid infection in transgenic plants expressing viroid-specific small RNAs derived from various functional domains

被引：24

作者：

Adkar-Purushothama, Charith Raj ^{[1
]}

Kasai, Atsushi ^{[1
]}

Sugawara, Kohei ^{[1
]}

Yamamoto, Hideki ^{[1
]}

Yamazaki, Yuto ^{[1
]}

He, Ying-Hong ^{[1
]}

Takada, Nobuyuki ^{[1
]}

Goto, Hideki ^{[1
]}

Shindo, Sahori ^{[1
]}

Harada, Takeo ^{[1
]}

Sano, Teruo ^{[1
]}

机构：

[1] Hirosaki Univ, Fac Agr & Life Sci, Hirosaki, Aomori 0368561, Japan

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

日本学术振兴会;

关键词：

VIRULENCE MODULATING REGION; SPINDLE; GENE; PATHOGENICITY; RESISTANCE; BIOLOGY; SIRNAS; VIRUS; FORMS;

D O I：

10.1038/srep17949

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Previous attempts to develop RNAi-mediated viroid-resistant transgenic plants using nearly full-length Potato spindle tuber viroid (PSTVd) hairpin RNA (hpRNA) were successful; however unusual phenotypes resembling viroid infection occurred. Therefore, in the present work, transgenic Nicotiana benthamiana lines expressing both partial and truncated versions of PSTVd hpRNA were developed. Specifically, seven partial or truncated versions of PSTVd sequences were selected according to the hotspots of both PSTVd-sRNAs and functional domains of the PSTVd. A total of 21 transgenic lines Nicotiana benthamiana were developed under the control of either the CaMV-35S or the CoYMV promoters. All of the transgenic lines established here were monitored for the induction of phenotypic changes, for PSTVd-sRNA expression and for the resistance against PSTVd infection. Additionally, this study demonstrates the use of inverted repeat construct sequences as short as 26- to -49 nucleotides for both the efficient expression of the PSTVd-sRNA and the inhibition of PSTVd infection.

引用

页数：13

共 39 条

[1] Small RNA Derived from the Virulence Modulating Region of the Potato spindle tuber viroid Silences callose synthase Genes of Tomato Plants [J].