Enzymatic synthesis of octyl glucoside catalyzed by almond β-glucosidase in organic media

被引：11

作者：

Ducret, A ^{[1
]}

Carrière, JF ^{[1
]}

Trani, M ^{[1
]}

Lortie, R ^{[1
]}

机构：

[1] Natl Res Council Canada, Biotechnol Res Inst, Microbial & Enzyme Technol Grp, Bioproc Sector, Montreal, PQ H4P 2R2, Canada

来源：

CANADIAN JOURNAL OF CHEMISTRY | 2002年 / 80卷 / 06期

关键词：

beta-glucosidase; alkyl glycosides; reverse hydrolysis; water activity; organic solvent;

D O I：

10.1139/V02-081

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis of n-octyl-beta-D-glucopyranoside can be performed by direct condensation of 1-octanol and glucose, catalyzed by immobilized almond beta-glucosidase, using solid glucose suspended in 1-octanol as a reaction media. Both the rate of reaction and the conversion could be enhanced by using acetonitrile and N,N-dimethylformamide (DMF) as co-solvents, the latter giving the best results. The rate of reaction was dependent on the concentration of DMF and on the initial water activity (a(w)), with higher water activity fostering faster reactions. The rate increased with DMF concentration, up to 20% DMF, but diminished rapidly at higher concentrations. Product concentration could be increased from 40 to 100 mM by going from 0 to 20% DMF; however, it was not sensitive to the initial water activity.

引用

页码：653 / 656

页数：4

共 25 条

[1] SOLVENT DIELECTRIC EFFECTS ON PROTEIN DYNAMICS [J].