Protective Effect of Cactus Cladode Extracts on Peroxisomal Functions in Microglial BV-2 Cells Activated by Different Lipopolysaccharides

被引：9
作者：
Saih, Fatima-Ezzahra [1 ,2 ,3 ]
Andreoletti, Pierre [1 ,2 ]
Mandard, Stephane [4 ,5 ]
Latruffe, Norbert [1 ,2 ]
El Kebbaj, M'Hammed Said [6 ]
Lizard, Gerard [1 ,2 ]
Nasser, Boubker [3 ]
Cherkaoui-Malki, Mustapha [1 ,2 ]
机构：
[1] Lab BioPeroxIL, EA 7270, Unite Format & Rech Sci Vie Terre & Environm, Biochim Peroxysome Inflammat & Metab Lipid, F-21000 Dijon, France
[2] Univ Bourgogne Franche Comte, Lab BioPeroxIL, 6 Bd Gabriel, F-21000 Dijon, France
[3] Univ Hassan I, Fac Sci & Tech, Lab Biochim & Neurosci, BP 577, Settat 26000, Morocco
[4] Univ Bourgogne Franche Comte, Lipness Team, INSERM, Res Ctr,UMR866, F-21079 Dijon, France
[5] Univ Bourgogne Franche Comte, LabEx LipSTIC, Fac Med, F-21079 Dijon, France
[6] Univ Hassan II Casablanca, Fac Sci Ben Msik, Lab Biol & Sante, Ave Cdt Driss El Harti BP 7955, Casablanca 20100, Morocco
来源：
MOLECULES | 2017年 / 22卷 / 01期
关键词：
acyl-CoA oxidase 1; catalase; -oxidation; Escherichia coli; lipopolysaccharides; LPS; nitric oxide; Opuntia; peroxisomes; Salmonella minnesota; OPUNTIA-FICUS-INDICA; NITRIC-OXIDE SYNTHASE; INFLAMMATORY MEDIATORS; INHIBITION; NEURODEGENERATION; EXPRESSION; SYSTEM; DAMAGE; HEALTH; INJURY;
D O I：
10.3390/molecules22010102
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we aimed to evaluate the antioxidant and anti-inflammatory properties of Opuntia ficus-indica cactus cladode extracts in microglia BV-2 cells. Inflammation associated with microglia activation in neuronal injury can be achieved by LPS exposure. Using four different structurally and biologically well-characterized LPS serotypes, we revealed a structure-related differential effect of LPS on fatty acid -oxidation and antioxidant enzymes in peroxisomes: Escherichia coli-LPS decreased ACOX1 activity while Salmonella minnesota-LPS reduced only catalase activity. Different cactus cladode extracts showed an antioxidant effect through microglial catalase activity activation and an anti-inflammatory effect by reducing nitric oxide (NO) LPS-dependent production. These results suggest that cactus extracts may possess a neuroprotective activity through the induction of peroxisomal antioxidant activity and the inhibition of NO production by activated microglial cells.
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页数：13
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共 50 条
[1]   An expeditious synthesis of spinasterol and schottenol, two phytosterols present in argan oil and in cactus pear seed oil, and evaluation of their biological activities on cells of the central nervous system [J].
Badreddine, Asmaa ;
El Mostafa, Karym ;
Zarrouk, Amira ;
Nury, Thomas ;
El Kharrassi, Youssef ;
Nasser, Boubker ;
Malki, Mustapha Cherkaoui ;
Lizard, Gerard ;
Samadi, Mohammad .
STEROIDS, 2015, 99 :119-124
[2]   Chronic microglial activation and progressive dopaminergic neurotoxicity [J].
Block, M. L. ;
Hong, J. -S. .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2007, 35 :1127-1132
[3]   EXPRESSION OF LIVER PEROXISOMAL PROTEINS AS COMPARED TO OTHER ORGANELLE MARKER ENZYMES IN RATS TREATED WITH HYPOLIPIDEMIC AGENTS [J].
CHERKAOUIMALKI, M ;
BARDOT, O ;
LHUGUENOT, JC ;
LATRUFFE, N .
BIOLOGY OF THE CELL, 1990, 69 (02) :83-92
[4]   Endotoxin induces structure-function alterations of rat liver peroxisomes: Kupffer cells released factors as possible modulators [J].
Contreras, MA ;
Khan, M ;
Smith, BT ;
Cimini, AM ;
Gilg, AG ;
Orak, J ;
Singh, I ;
Singh, AK .
HEPATOLOGY, 2000, 31 (02) :446-455
[5]   Microglia and neurodegeneration: The role of systemic inflammation [J].
Cunningham, Colm .
GLIA, 2013, 61 (01) :71-90
[6]   RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY [J].
DENIZOT, F ;
LANG, R .
JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 89 (02) :271-277
[7]   IMPAIRMENT OF PEROXISOMAL BETA-OXIDATION SYSTEM BY ENDOTOXIN TREATMENT [J].
DHAUNSI, GS ;
HANEVOLD, CD ;
SINGH, I .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 1994, 135 (02) :187-193
[8]   The Inflammatory Response in Acyl-CoA Oxidase 1 Deficiency (Pseudoneonatal Adrenoleukodystrophy) [J].
El Hajj, H. I. ;
Vluggens, A. ;
Andreoletti, P. ;
Ragot, K. ;
Mandard, S. ;
Kersten, S. ;
Waterham, H. R. ;
Lizard, G. ;
Wanders, R. J. A. ;
Reddy, J. K. ;
Cherkaoui-Malki, Mustapha .
ENDOCRINOLOGY, 2012, 153 (06) :2568-2575
[9]  
El Kebbaj Riad, 2015, Biochim Open, V1, P51, DOI 10.1016/j.biopen.2015.10.002
[10]  
El-Kebbaj R., 2013, Health, V5, P62, DOI 10.4236/health.2013.51009
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