Shuttling Gold Nanoparticles into Tumoral Cells with an Amphipathic Proline-Rich Peptide

被引:44
作者
Pujals, Silvia [1 ]
Bastus, Neus G. [2 ]
Pereiro, Eva [3 ]
Lopez-Iglesias, Carmen [4 ]
Punte, Victor F. [2 ]
Kogan, Marcelo J. [5 ]
Giralt, Ernest [1 ,6 ]
机构
[1] Inst Recerca Biomed, Barcelona 08028, Spain
[2] Inst Catala Nanotecnol, Barcelona 08193, Spain
[3] ALBA Synchrotron Light Source, Barcelona 08193, Spain
[4] Univ Barcelona, Unitat Microscopia Elect & Reconeixement Mol, Situ Serv Cient Tecn, E-08028 Barcelona, Spain
[5] Univ Chile, Dept Pharmacol & Toxicol Chem, Ctr Invest Interdisciplinaria Avanzada Ciencia Ma, Santiago 1007, Chile
[6] Univ Barcelona, Dept Quim Organ, E-08028 Barcelona, Spain
关键词
cell-penetrating peptides; drug delivery; gold nanoparticles; nanostructures; proline; ENGINEERED INORGANIC NANOPARTICLES; X-RAY TOMOGRAPHY; CARBON NANOSTRUCTURES; CARRIERS; DNA; NANOCRYSTALS; COMPLEXES; PROTEINS; CONTRAST; DELIVERY;
D O I
10.1002/cbic.200800843
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell-penetrating peptides (CPPs) are a potential tool for intracellular delivery of different kinds of cargoes. Because of their growing use in nanobiomedicine, both for diagnostics and for treatment, metal nanoparticles are an interesting cargo for CPPs. Here, gold nanoparticles (AuNps) and the amphipathic proline-rich peptide SAP have been used. Conjugation of the peptide onto the AuNps was achieved by addition of a cysteine to the SAP sequence for thiol chemisorption on gold, and the attachment was confirmed by visible spectroscopy, dynamic light scattering (DLS), zeta-potential (ZP), stability towards ionic strength (as high as 1 m NaCl), X-ray photoelectron spectroscopy (XPS) and high-resolution transmission electron microscopy (HR-TEM) coupled to electron energy loss spectroscopy (EELS). AuNp-C-SAP internalization in HeLa cells was observed by three different microscopy techniques-TEM, confocal laser scanning microscopy (CLSM) and transmission X-ray microscopy (TXM)-and all of them have confirmed the effective intracellular delivery of AuNps by SAP.
引用
收藏
页码:1025 / 1031
页数:7
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