Genetic variation of high-risk HPV-16 from a cervical cancer biopsy collected in Pakistan

被引：2

作者：

Siddiqa, Abida ^{[1
]}

Bhatti, Muhammad Faraz ^{[1
]}

机构：

[1] NUST, Atta ur Rahman Sch Appl Biosci ASAB, Sect H12, Islamabad 44000, Pakistan

来源：

FUTURE VIROLOGY | 2016年 / 11卷 / 01期

关键词：

cervical cancer; genetic variation; Pakistan; papillomavirus; HUMAN-PAPILLOMAVIRUS TYPE-16; NUCLEOTIDE-SEQUENCE ANALYSIS; HUMAN KERATINOCYTES; MOLECULAR VARIANTS; REGULATORY REGION; LIFE-CYCLE; DNA; E2; E6; TRANSCRIPTION;

D O I：

10.2217/fvl.15.94

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim: HPV-16 is one of the causative agents of cervical cancer. The HPV-16 genome is characterized widely to assist mutagenesis studies, phylogenetic analysis and protein annotations. This is the first report of HPV-16 sequence analysis from Pakistan. Materials & methods: The gene and long control region specific primers were used to generate the targeted amplicons that were then cloned and sequenced. Results: The complete genome analysis revealed nine changes both at amino acid and nucleotide level including seven novel and two previously reported mutations. The evolutionary relationship of HPV from Pakistan and reported sequences shows that strain reported in this study segregates with European taxa. Conclusion: This report lays foundation to conduct HPV genome mutation analysis in Pakistan.

引用

页码：31 / 38

页数：8

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