CDX2 is essential for cell proliferation and polarity in porcine blastocysts

被引：67

作者：

Bou, Gerelchimeg ^{[1
,2
]}

Liu, Shichao ^{[1
]}

Sun, Mingju ^{[1
]}

Zhu, Jiang ^{[1
]}

Xue, Binghua ^{[1
]}

Guo, Jia ^{[1
]}

Zhao, Yueming ^{[1
]}

Qu, Bo ^{[3
]}

Weng, Xiaogang ^{[1
]}

Wei, Yanchang ^{[1
]}

Lei, Lei ^{[4
]}

Liu, Zhonghua ^{[1
,5
]}

机构：

[1] Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

[2] Inner Monaolia Agr Univ, Coll Anim Sci, Hohhot 010018, Peoples R China

[3] Northeast Agr Univ, Life Sci & Biotech Res Ctr, Harbin 150030, Peoples R China

[4] Harbin Med Univ, Dept Histol & Embryol, Harbin 150081, Peoples R China

[5] Dept Heilongjiang Prov, Key Lab Anim Genet Breeding & Reprod, Harbin 150030, Peoples R China

来源：

DEVELOPMENT | 2017年 / 144卷 / 07期

基金：

中国国家自然科学基金;

关键词：

CDX2; TE polarity; Cell proliferation; BMP4; PKC alpha; EARLY LINEAGE SEGREGATION; DEVELOPMENT IN-VITRO; PREIMPLANTATION DEVELOPMENT; MOUSE EMBRYO; EXPRESSION PATTERNS; PRIMITIVE ENDODERM; GENE-EXPRESSION; PROVIDED CDX2; MESSENGER-RNA; MATERNAL CDX2;

D O I：

10.1242/dev.141085

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The role of CDX2 in trophectoderm (TE) cells has been extensively studied, yet the results are contradictory and species specific. Here, CDX2 expression and function were explored in early porcine embryos. Notably, siRNA-mediated gene knockdown and lentivirus-mediated TE-specific gene regulation demonstrated that CDX2 is essential for the maintenance of blastocyst integrity by regulating the BMP4-mediated blastocyst niche and classic protein kinase C (PKC)-mediated TE polarity in mammalian embryos. Mechanistically, CDX2-depleted porcine embryos stalled at the blastocyst stage and exhibited apoptosis and inactive cell proliferation, possibly resulting from BMP4 downregulation. Moreover, TE cells in CDX2-depleted blastocysts displayed defective F-actin apical organization associated with downregulation of PKC alpha (PRKCA). Collectively, these results provide further insight into the functional diversity of CDX2 in early mammalian embryos.

引用

页码：1296 / 1306

页数：11

共 56 条

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