Complete mitochondrial DNA sequence of the Japanese spiny lobster, Panulirus japonicus (Crustacea: Decapoda)

被引:83
作者
Yamauchi, MM
Miya, MU
Nishida, M
机构
[1] Univ Tokyo, Ocean Res Inst, Nakano Ku, Tokyo 1648639, Japan
[2] Nat Hist Museum & Inst, Dept Zool, Chuo Ku, Chiba 2608682, Japan
关键词
mitochondrial genome; long polymerase chain reaction; decapod phylogeny;
D O I
10.1016/S0378-1119(02)00824-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We determined the complete nucleotide sequence of the mitochondrial genome for a Japanese spiny lobster, Panulirus japonicus (Crustacea: Decapoda). The entire genome was amplified using long polymerase chain reaction, and the products were subsequently used as templates for direct sequencing using a primer-walking strategy. The genome (15,717 base pairs) contained the same 37 genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) plus the putative control region as found in other arthropods, with the gene order identical to that of typical arthropods. Preliminary phylogenetic analyses of selected arthropods using concatenated amino acid sequences of the 13 protein-coding genes strongly supported monophyly of Decapoda species and confidently rejected 'Macroura', a conventional taxon that shares an elongated abdominal body. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:89 / 96
页数:8
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