Exiguobacterium flavidum sp. nov., isolated from the Red Maple Lake

A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China. The DNA G+C content of the strain HF6OT was 55.0%. The predominant isoprenoid quinones were identified as MK-7 (56.4%) and MK-8 (35.7%). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophosphoglycolipid. The major fatty acids were anteiso-C-13:0, iso-C-15(:0), C-16(:0) and iso-C-13:0. The strain had cell wall peptidoglycan type A3 alpha L-Lys-Gly. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60(T) belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490(T) (97.2% 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481(T) (97.1 %), Exiguobacterium antarcticum DSM 14480(T) (96.9%) and Exiguobacterium aurantiacum NBRC 14763(T) (94.5%). The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60(T) can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp. nov. is proposed, The type strain is HF60(T) (=MCCC 1H00336(T)=KCTC 33987(T)).