Comparative Study of Amplification Systems in Immunoenzyme Assays for the Diagnosis of American Tegumentary Leishmaniasis

被引:4
作者
Nascimento, Lilian Dias [1 ]
Lambert Passos, Sonia Regina [2 ]
Mouta-Confort, Eliame [1 ]
Santiago, Marta de Almeida [3 ]
Alves, Andreia Silva [1 ]
Madeira, Maria de Fatima [1 ]
Schubach, Armando de Oliveira [1 ]
de Almeida Marzochi, Mauro Celio [1 ]
机构
[1] Fiocruz MS, Lab Vigilancia Leishmanioses, Inst Pesquisa Clin Evandro Chagas, BR-21040960 Rio De Janeiro, Brazil
[2] Fiocruz MS, Lab Epidemiol Clin, Inst Pesquisa Clin Evandro Chagas, BR-21040960 Rio De Janeiro, Brazil
[3] Fiocruz MS, Lab Tecnol Diagnost, Inst Tecnol Imunobiol Biomanguinhos, BR-21040960 Rio De Janeiro, Brazil
关键词
American tegumentary leishmaniasis; Leishmania (Viannia) braziliensis; immunoassay; amplification systems; LINKED-IMMUNOSORBENT-ASSAY; ENZYME-IMMUNOASSAY; CUTANEOUS LEISHMANIASIS; ELISA; SERODIAGNOSIS; ANTIGEN; SENSITIVITY; ANTIBODIES;
D O I
10.1002/jcla.20308
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
We compared the accuracy and reliability of three amplification systems for enzyme immunoassays in the detection of specific IgG antibodies for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in patients from an endemic area in Rio de Janeiro, Brazil. Partially soluble antigens obtained from the promastigote forms of L. (V.) braziliensis were used. For development of the reaction, two chromogens, 1,2-orthophenylenediamine (OPD) and 3,3',5,5'-tetramethylbenzidine (TMB), and a fluorogen, 4-methylumbelliferyl phosphate (MUP), were tested. The performance of each system was compared using the following parameters: accuracy, intraclass correlation coefficient (ICC), and area under the receiver operating characteristic (ROC) curve. Sensitivity was the same (97.4%) for all systems. The reliability was excellent (ICC = 98.6, 98.7, and 99.1%) and specificity was 93.7, 95.8, and 97.4% for OPD, MUP, and TMB, respectively, showing no statistical significance. Despite the absence of differences in the performance of the three systems, the use of TMB is suggested because of its operational advantages, such as low cost compared with fluorogens, easy manipulation, greater stability, and lower toxicity. J. Clin. Lab. Anal. 23:152-156, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:152 / 156
页数:5
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