Effect of umbilical cord serum coated 3D PCL/alginate scaffold for mastoid obliteration

被引：20
|
作者：
Jang, Chul Ho [1 ,2 ]
Cho, Yong Beom [1 ]
Choi, Cheol Hee [2 ,3 ]
Jang, Yoon Seok [3 ]
Jung, Won-Kyo [4 ,5 ]
Lee, Hyeongjin [6 ]
Kim, Geun Hyung [6 ]
机构：
[1] Chonnam Natl Univ, Dept Otolaryngol, Sch Med, Kwangju, South Korea
[2] Chosun Univ, Res Ctr Resistant Cells, Kwangju, South Korea
[3] Chosun Univ, Dept Bio New Drug Dev, Kwangju, South Korea
[4] Pukyong Natl Univ, Dept Biomed Engn, Pusan, South Korea
[5] Pukyong Natl Univ, Ctr Marine Integrated Biomed Technol BK Plus 21, Pusan, South Korea
[6] Sungkyunkwan Univ, Dept Biomechatron Engn, Coll Biotechnol & Bioengn, Suwon, South Korea
来源：
INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | 2014年 / 78卷 / 07期
关键词：
Mastoid obliteration; Polycaprolactone; Umbilical cord serum; Osteogenesis; PLATELET-RICH PLASMA; IN-VITRO; EPITHELIAL DEFECTS; BONE REGENERATION; COMBINATION; THERAPY; CELLS; COMPOSITE; EYEDROPS;
D O I：
10.1016/j.ijporl.2014.04.004
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Objective: Human umbilical cord serum (hUCS) has a lot of growth factors. To date, there are no reports on stimulating effect of hUCS in osteogenesis. The purpose of this study is to evaluate enhancing effect of hUCS in osteogenesis by mastoid obliteration combined with bony scaffold. Materials and methods: The fabrication procedure for obtaining PCL/alginate/hUCS was performed. The bulla obliteration was done using PCL/alginate/hUCS in the experimental group and PCL in the control group. To assess the early active mineralization of new bone formation, guinea pigs of each group received an intraperitoneal infusion with alizarin red at 6 weeks post-surgery. The animals of each group were sacrificed 8 weeks post-surgery. Ex vivo microCT and histologic observation were performed. Results: MicroCT finding shows more radiopaque change within the pores in the experimental group compared to the control group. Stereomicroscopic and SEM findings show new bone formation of the pores in the experimental group. However, the pores between strands almost all remained in the control group. Corresponding histological observations for the stimulatory effects of hUCS showed osteogenesis in the pores between the strands compared to the control group. Conclusion: Our data suggest that hUCS coated 3D porous PCL scaffold in mastoid obliteration provides enhanced osteogenesis. Therefore, we suggest that our hUCS coated 3D porous PCL could be used in mastoid obliteration in the future. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：1061 / 1065
页数：5
相关论文
共 50 条
  • [21] Study on the fabrication and performance of hierarchical porous 3D printed PCL-based artificial bone scaffold with anti-bacterial effect
    Liang, Junjie
    Lian, Xiaojie
    Lu, Yi
    Feng, Haonan
    Qin, Tingwei
    Lei, Qi
    Zhang, Siruo
    Liang, Ziwei
    Huang, Di
    MATERIALS TODAY COMMUNICATIONS, 2024, 39
  • [22] Enhanced osteogenic differentiation of stem cells by 3D printed PCL scaffolds coated with collagen and hydroxyapatite
    Ebrahimi, Zahra
    Irani, Shiva
    Ardeshirylajimi, Abdolreza
    Seyedjafari, Ehsan
    SCIENTIFIC REPORTS, 2022, 12 (01)
  • [23] Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
    Tian, Lijiao
    Zhang, Zhenting
    Tian, Bin
    Zhang, Xin
    Wang, Na
    RSC ADVANCES, 2020, 10 (08) : 4805 - 4816
  • [24] Fabrication of Polycaprolactone/Nano Hydroxyapatite (PCL/nHA) 3D Scaffold with Enhanced In Vitro Cell Response via Design for Additive Manufacturing (DfAM)
    Cho, Yong Sang
    Gwak, So-Jung
    Cho, Young-Sam
    POLYMERS, 2021, 13 (09)
  • [25] Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering
    Wang, Limin
    Singh, Milind
    Bonewald, Lynda F.
    Detamore, Michael S.
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2009, 3 (05) : 398 - 404
  • [26] A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair
    Li, Hehong
    Yin, Yingxian
    Xiang, Yang
    Liu, Haixia
    Guo, Rui
    BIOMEDICAL MATERIALS, 2020, 15 (04)
  • [27] 3D printed PCL scaffold reinforced with continuous biodegradable fiber yarn: A study on mechanical and cell viability properties
    Hedayati, Seyyed Kaveh
    Behravesh, Amir Hossein
    Hasannia, Sadegh
    Saed, Arvin Bagheri
    Akhoundi, Behnam
    POLYMER TESTING, 2020, 83 (83)
  • [28] Onlay-graft of 3D printed Kagome-structure PCL scaffold incorporated with rhBMP-2 based on hyaluronic acid hydrogel
    Ku, Jeong-Kui
    Lee, Kang-Gon
    Ghim, Min-Soo
    Kim, Young-Kyun
    Park, Sang-Hyug
    Park, Yongdoo
    Cho, Young-Sam
    Lee, Bu-Kyu
    BIOMEDICAL MATERIALS, 2021, 16 (05)
  • [29] In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation
    Amaral, Danielle L. A. S.
    Zanette, Rafaella S. S.
    Almeida, Camila G.
    Almeida, Leonara B. F.
    de Oliveira, Luiz F. C.
    Marcomini, Raphael F.
    Nogueira, Breno, V
    Santos, Marcelo O.
    Brandao, Humberto M.
    Maranduba, Carlos M. C.
    Munk, Michele
    BIOMEDICAL MATERIALS, 2019, 14 (03)
  • [30] Thermogelable PNIPAM microgel dispersion as 3D cell scaffold: effect of syneresis
    Gan, Tiantian
    Guan, Ying
    Zhang, Yongjun
    JOURNAL OF MATERIALS CHEMISTRY, 2010, 20 (28) : 5937 - 5944
12345