Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin

被引：58
|
作者：
Kim, Sung Eun [1 ,2 ]
Kim, Chang-Seop [3 ]
Yun, Young-Pil [1 ,2 ]
Yang, Dae Hyeok [4 ]
Park, Kyeongsoon [5 ]
Kim, Se Eun [6 ]
Jeong, Chang-Mo [3 ]
Huh, Jung-Bo [3 ]
机构：
[1] Korea Univ, Med Ctr, Guro Hosp, Dept Orthoped Surg, Seoul 152703, South Korea
[2] Korea Univ, Med Ctr, Guro Hosp, Rare Dis Inst, Seoul 152703, South Korea
[3] Pusan Natl Univ, Sch Dent, Dent Res Inst, Dept Prosthodont, Yang San 626787, South Korea
[4] Catholic Univ Korea, Coll Med, Inst Cell & Tissue Engn, Seoul 137701, South Korea
[5] Korea Basic Sci Inst, Div Bioimaging, Chunchon 200701, Gangwon Do, South Korea
[6] Chonnam Natl Univ, Coll Vet Med, Dept Vet Surg, Kwangju 500757, South Korea
来源：
CARBOHYDRATE POLYMERS | 2014年 / 114卷
基金：
新加坡国家研究基金会;
关键词：
Titanium (Ti); Bone morphogenetic protein-2 (BMP-2); Heparin; Osteoblast function; Bone formation; MORPHOGENIC PROTEIN-2; GROWTH-FACTOR; STEM-CELLS; IMPLANTS; REGENERATION; HYDROGEL; COATINGS; ADHESION; DELIVERY; COLLAGEN;
D O I：
10.1016/j.carbpol.2014.08.005
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：123 / 132
页数：10
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