Use of Formalin-Fixed Paraffin-Embedded Tissue and Single-Strand Conformation Polymorphism Analysis for Polymerase Chain Reaction of Antigen Receptor Rearrangements in Dogs

被引：5

作者：

Kaneko, Naoki ^{[1
]}

Tanimoto, Takashi ^{[1
]}

Morimoto, Masahiro ^{[2
]}

Hayashi, Toshiharu ^{[2
]}

Miyamao, Takako Shimokawa ^{[1
]}

Hiraoka, Hiroko ^{[1
]}

Itamoto, Kazuhito ^{[3
]}

Une, Satoshi ^{[4
]}

Mizuno, Takuya ^{[1
]}

Okuda, Masaru ^{[1
]}

机构：

[1] Yamaguchi Univ, Fac Agr, Lab Vet Internal Med, Yamaguchi 7538515, Japan

[2] Yamaguchi Univ, Fac Agr, Lab Vet Pathol, Yamaguchi 7538515, Japan

[3] Yamaguchi Univ, Fac Agr, Lab Vet Surg, Yamaguchi 7538515, Japan

[4] Yamaguchi Univ, Fac Agr, Lab Vet Clin Diag, Yamaguchi 7538515, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2009年 / 71卷 / 04期

关键词：

cancer diagnosis; canine; lymphoma/leukemia; PCR; tumor diagnosis; LYMPHOMA; MONOCLONALITY; DIAGNOSIS; DNA;

D O I：

10.1292/jvms.71.535

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was Successful in only three of five samples. The formalin Fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls Suggested that a formalin fixation time of less than one Week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin Fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia.

引用

页码：535 / 538

页数：4

共 13 条

[1] Diagnosis of canine lymphoid neoplasia using clonal rearrangements of antigen receptor genes [J].

Burnett, RC ;

Vernau, W ;

Modiano, JF ;

Olver, CS ;

Moore, PF ;

Avery, AC .

VETERINARY PATHOLOGY, 2003, 40 (01) :32-41

[2]

Camilleri-Broet S, 2000, Ann Diagn Pathol, V4, P71, DOI 10.1016/S1092-9134(00)90014-5

[3] Semireannealing, single-stranded conformational polymorphism: A novel and effective tool for the diagnosis of T-cell clonality [J].

Crisi, GM ;

Emanuel, JR ;

Johnson, C ;

Crotty, P ;

Costa, J ;

Tallini, G .

DIAGNOSTIC MOLECULAR PATHOLOGY, 2002, 11 (02) :67-74

[4] SOURCES OF DNA FOR DETECTING B-CELL MONOCLONALITY USING PCR [J].

DISS, T ;

PAN, L ;

PENG, H ;

WOTHERSPOON, AC ;

ISAACSON, PG .

JOURNAL OF CLINICAL PATHOLOGY, 1994, 47 (06) :493-496

[5]

Han XQ, 2004, WORLD J GASTROENTERO, V10, P995

[6] Hodgkin's lymphoma of T-cell type: Clonal association with a CD30+cutaneous lymphoma [J].

Kadin, ME ;

Drews, R ;

Samel, A ;

Gilchrist, A ;

Kocher, O .

HUMAN PATHOLOGY, 2001, 32 (11) :1269-1272

[7]

Kaneko N, 2005, Vet Comp Oncol, V3, P203, DOI 10.1111/j.1476-5810.2005.00080.x

[8] Application of laser capture microdissection to cytologic specimens for the detection of immunoglobulin heavy chain gene rearrangement in patients with malignant lymphoma [J].

Orba, Y ;

Tanaka, S ;

Nishihara, H ;

Kawamura, N ;

Itoh, T ;

Shimizu, M ;

Sawa, H ;

Nagashima, K .

CANCER CYTOPATHOLOGY, 2003, 99 (04) :198-204

[9] DETECTION OF POLYMORPHISMS OF HUMAN DNA BY GEL-ELECTROPHORESIS AS SINGLE-STRAND CONFORMATION POLYMORPHISMS [J].

ORITA, M ;

IWAHANA, H ;

KANAZAWA, H ;

HAYASHI, K ;

SEKIYA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2766-2770

[10] Detection of clonal T-cell receptor γ gene rearrangements in paraffin-embedded tissue by polymerase chain reaction and nonradioactive single-strand conformational polymorphism analysis [J].

Signoretti, S ;

Murphy, M ;

Cangi, MG ;

Puddu, P ;

Kadin, ME ;

Loda, M .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (01) :67-75

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