Extraction, characterization and antioxidant activities of polysaccharides from E. corneum gigeriae galli

被引：48

作者：

Xiong, Qingping ^{[1
,4
]}

Li, Xia ^{[2
]}

Zhou, Ruizhen ^{[3
]}

Hao, Hairong ^{[2
]}

Li, Songlin ^{[1
]}

Jing, Yi ^{[1
]}

Zhu, Chun ^{[1
,4
]}

Zhang, Qinghua ^{[1
]}

Shi, Yingying ^{[1
]}

机构：

[1] Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian 223001, Jiangsu, Peoples R China

[2] Xuzhou Med Coll, Affiliated Huaian Hosp, Huaian 223002, Jiangsu, Peoples R China

[3] Guangzhou Univ Chinese Med, Sch Basic Med, Guangzhou 510006, Guangdong, Peoples R China

[4] Huaiyin Inst Technol, Jiangsu Prov Engn Lab Biomass Convers & Proc Inte, Huaian 223003, Jiangsu, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 108卷

关键词：

E. corneum gigeriae galli; Polysaccharide; Enzyme-assisted extraction; Characterization; Antioxidant activity; RESPONSE-SURFACE METHODOLOGY; OPTIMIZATION; PURIFICATION; COMPONENTS;

D O I：

10.1016/j.carbpol.2014.02.068

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In the present study, optimization of enzyme-assisted extraction, characterization and antioxidant activities in vitro of polysaccharides from Endothelium corneum gigeriae galli (PEGG) were investigated. It was found that the optimum extraction conditions were determined as follows: extraction temperature 87.0 degrees C, extraction time 177.0 min, enzyme concentration 1.65%, enzymatic hydrolysis time 141.0 min, liquid-to-solid ratio 20, enzymatic hydrolysis temperature 55 degrees C and enzymatic hydrolysis pH 3.6. Under these conditions, the experimental yield of polysaccharides was 5.08%. In addition, PEGG had a relatively high sulfate radical content. PEGG was composed of rhamnose, fucose, mannose, glucose and galactose, with molar percentages of 13.1, 4.5, 72.8 and 9.6%, respectively.The average molecular weight was 83 kDa. And there were infrared characteristic absorption peaks of polysaccharides in the FT-IR spectroscopy of PEGG. For antioxidant activities in vitro, PEGG showed possessed strong hydroxyl radical scavenging, Fe2+ chelating and lipid peroxidation inhibitory activities. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：247 / 256

页数：10

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