Elevated expression of TGIF is involved in lung carcinogenesis

被引：17

作者：

Wang, Yadong ^{[1
]}

Wang, Haiyu ^{[1
]}

Gao, Huiyan ^{[1
]}

Xu, Bing ^{[1
]}

Zhai, Wenlong ^{[2
]}

Li, Jiangmin ^{[1
]}

Zhang, Congke ^{[1
]}

机构：

[1] Henan Ctr Dis Control & Prevent, Dept Toxicol, Zhengzhou 450016, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Gen Surg, Zhengzhou 450052, Peoples R China

来源：

TUMOR BIOLOGY | 2015年 / 36卷 / 12期

基金：

中国国家自然科学基金;

关键词：

TGIF; Lung carcinogenesis; Benzo(a)pyrene; Lung cancer; INTERACTING-FACTOR; HEPATOCELLULAR-CARCINOMA; CANCER; EXPOSURE; CELLS; POLYMORPHISMS; PROFILES; PROTEIN; SMAD2; RISK;

D O I：

10.1007/s13277-015-3615-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The purpose of this study was to explore the expression of TG-interacting factor (TGIF) in lung carcinogenesis. Malignant transformation of human bronchial epithelial (16HBE) cell was established by benzo(a)pyrene (BaP) treatment. Soft agar assay and tumor formation assay in nude mice were applied. Tumorigenesis experiment in vivo was done by BaP treatment. Western blotting, immunohistochemistry, and quantitative polymerase chain reaction were used to detect TGIF expression. We observed a higher level of TGIF messenger RNA (mRNA) in lung cancer tissues than that in paracancerous tissues. We observed significantly higher levels of TGIF mRNA and protein in A549 and H1299 cell lines than that in 16HBE cell. Increased expressions of TGIF protein and mRNA were observed in 16HBE cells induced by BaP treatment as compared to those in solvent control group. We observed significantly higher levels of TGIF mRNA and protein in 16HBE-BaP cells than that in 16HBE-control cells. We observed significantly higher levels of TGIF mRNA and protein in mice lung tissues treated with BaP than that in control group. Our results suggested that elevated expression of TGIF was involved in lung carcinogenesis.

引用

页码：9223 / 9231

页数：9

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