Effect of timing of collection of salivary metabolomic biomarkers on oral cancer detection

被引：57

作者：

Ishikawa, Shigeo ^{[1
]}

Sugimoto, Masahiro ^{[2
,3
]}

Kitabatake, Kenichiro ^{[1
]}

Tu, Micheal ^{[4
]}

Sugano, Ayako ^{[1
]}

Yamamori, Iku ^{[1
]}

Iba, Asuka ^{[1
]}

Yusa, Kazuyuki ^{[1
]}

Kaneko, Miku ^{[2
]}

Ota, Sana ^{[2
]}

Hiwatari, Kana ^{[2
]}

Enomoto, Ayame ^{[2
]}

Masaru, Tomita ^{[2
]}

Iino, Mitsuyoshi ^{[1
]}

机构：

[1] Yamagata Univ, Fac Med, Dept Dent Oral & Maxillofacial Plast & Reconstruc, 2-2-2 Iida Nishi, Yamagata 9909585, Japan

[2] Keio Univ, Inst Adv Biosci, 246-2 Mizukami, Tsuruoka, Yamagata 9970052, Japan

[3] AMED, AMED CREST, Chiyoda Ku, 1-7-1 Otemachi, Tokyo 1000004, Japan

[4] Univ Calif Los Angeles, Sch Dent, 10833 Le Conte Ave, Los Angeles, CA 90095 USA

来源：

AMINO ACIDS | 2017年 / 49卷 / 04期

关键词：

Metabolomics; Oral cancer; Saliva; Collection condition; PRIMARY SJOGRENS-SYNDROME; SQUAMOUS-CELL CARCINOMA; VALIDATION; MEDICINE;

D O I：

10.1007/s00726-017-2378-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study is to evaluate the effect of duration after meals for saliva collections for oral cancer detection using metabolomics. Saliva samples were collected from oral cancer patients (n = 22) and controls (n = 44). Saliva from cancer patients was collected 12 h after dinner, and 1.5 and 3.5 h after breakfast. Control subjects fasted > 1.5 h prior to saliva collection. Hydrophilic metabolites were analyzed using capillary electrophoresis mass spectrometry. Levels of 51 metabolites differed significantly in controls vs. oral cancer patients at the 12-h fasting time point (P < 0.05). Fifteen and ten metabolites differed significantly at the 1.5- and 3.5-h time points, respectively. The area of under receiver operating characteristic curve for discriminating oral cancer patients from controls was greatest at the 12-h fasting time point. The collection time after meals affects levels of salivary metabolites for oral cancer screening. The 12-h fasting after dinner time point is optimal. This study contributes to design of saliva collection protocols for metabolomics-based biomarker discovery.

引用

页码：761 / 770

页数：10

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