DEVELOPMENT AND CHARACTERIZATION OF 25 EST-SSR MARKERS IN PINUS SYLVESTRIS VAR. MONGOLICA (PINACEAE)

被引：13
|
作者：
Fang, Pan [1 ]
Ni, Shihui [1 ]
Yuan, Huwei [1 ]
Li, Zhexin [1 ]
Zhan, Yuncheng [1 ]
Yuan, Lu [1 ]
Li, Wei [1 ]
机构：
[1] Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Natl Engn Lab Forest Tree Breeding, Minist Educ,Coll Biol Sci & Technol, Beijing 100083, Peoples R China
来源：
APPLICATIONS IN PLANT SCIENCES | 2014年 / 2卷 / 01期
基金：
中国国家自然科学基金;
关键词：
expressed sequence tag; Pinaceae; Pious sylvestris var. mongolica; polymorphism; primer pairs; transcriptome sequencing; L;
D O I：
10.3732/apps.1300057
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: A set of novel expressed sequence tag (EST) microsatelhte markers was developed in Pious sylvestris var, mongolica to promote further genetic studies in this species. Methods and Results: One hundred seventy-live EST simple sequence repeat (SSR) primers were designed and synthesized for 31,653 isotigs based on P. tabulifolmis EST sequences. The primer pairs were used to identify 25 polymorphic loci in 48 individuals. The number of alleles ranged from two to eight with observed and expected haerozygosity values of 0.0435 to 0.8125 and 0.0430 to 0.7820, respectively. Conclusions: These new polymorphic EST-SSR markers will be useful for assessing genetic diversity, molecular breeding and genetic improvement, and conservation of P. sylyestris var. mongolica.
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页数：4
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