Frozen tissue biobanks.: Tissue handling, cryopreservation, extraction, and use for proteomic analysis

被引：38
作者：
Ericsson, Christer
Franzen, Bo
Nister, Monica
机构：
[1] Karolinska Univ Hosp, Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden
[2] AstraZeneca, DMPK Res & Biomarkers, Res Area CNS & Pain Control, SE-15185 Sodertalje, Sweden
来源：
ACTA ONCOLOGICA | 2006年 / 45卷 / 06期
关键词：
D O I：
10.1080/02841860600818047
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The potential and the limitations of protein analysis of tissue samples are surveyed. The complexity, concentration range and dynamics of the human proteome are reviewed, as is the effect of handling and cryopreservation. Protein extraction, solubilization, resolution and detection are discussed, in relation to the properties of the human proteome.
引用
收藏
页码：643 / 661
页数：19
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