Frozen tissue biobanks.: Tissue handling, cryopreservation, extraction, and use for proteomic analysis

被引：38
作者：
Ericsson, Christer
Franzen, Bo
Nister, Monica
机构：
[1] Karolinska Univ Hosp, Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden
[2] AstraZeneca, DMPK Res & Biomarkers, Res Area CNS & Pain Control, SE-15185 Sodertalje, Sweden
来源：
ACTA ONCOLOGICA | 2006年 / 45卷 / 06期
关键词：
D O I：
10.1080/02841860600818047
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The potential and the limitations of protein analysis of tissue samples are surveyed. The complexity, concentration range and dynamics of the human proteome are reviewed, as is the effect of handling and cryopreservation. Protein extraction, solubilization, resolution and detection are discussed, in relation to the properties of the human proteome.
引用
收藏
页码：643 / 661
页数：19
相关论文
共 100 条
  • [1] Adam BL, 2002, CANCER RES, V62, P3609
  • [2] Toward a human blood serum proteome - Analysis by multidimensional separation coupled with mass spectrometry
    Adkins, JN
    Varnum, SM
    Auberry, KJ
    Moore, RJ
    Angell, NH
    Smith, RD
    Springer, DL
    Pounds, JG
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (12) : 947 - 955
  • [3] A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard
    Alban, A
    David, SO
    Bjorkesten, L
    Andersson, C
    Sloge, E
    Lewis, S
    Currie, I
    [J]. PROTEOMICS, 2003, 3 (01) : 36 - 44
  • [4] Proteomic characterization of the human centrosome by protein correlation profiling
    Andersen, JS
    Wilkinson, CJ
    Mayor, T
    Mortensen, P
    Nigg, EA
    Mann, M
    [J]. NATURE, 2003, 426 (6966) : 570 - 574
  • [5] The human plasma proteome - History, character, and diagnostic prospects
    Anderson, NL
    Anderson, NG
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (11) : 845 - 867
  • [6] Mass spectrometric quantitation of peptides and proteins using stable isotope standards and capture by anti-peptide antibodies (SISCAPA)
    Anderson, NL
    Anderson, NG
    Haines, LR
    Hardie, DB
    Olafson, RW
    Pearson, TW
    [J]. JOURNAL OF PROTEOME RESEARCH, 2004, 3 (02) : 235 - 244
  • [7] QUALITATIVE AND QUANTITATIVE STUDIES ON HUMAN MYELIN BASIC-PROTEIN INSITU WITH RESPECT TO TIME INTERVAL BETWEEN DEATH AND AUTOPSY
    ANSARI, KA
    RAND, A
    HENDRICKSON, H
    BENTLEY, MD
    [J]. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1976, 35 (02) : 180 - 190
  • [8] Influences of blood sample processing on low-molecular-weight proteome identified by surface-enhanced laser desorption/ionization mass spectrometry
    Banks, RE
    Stanley, AJ
    Cairns, DA
    Barrett, JH
    Clarke, P
    Thompson, D
    Selby, PJ
    [J]. CLINICAL CHEMISTRY, 2005, 51 (09) : 1637 - 1649
  • [9] STUDIES OF HUMAN MYELIN PROTEINS DURING OLD-AGE
    BERLET, HH
    VOLK, B
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 1980, 14 (1-2) : 211 - 222
  • [10] BILD AH, 2005, NATURE
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