Protective effect of galangin against dextran sulfate sodium (DSS)-induced ulcerativecolitis in Balb/c mice

被引:57
|
作者
Sangaraju, Rajendra [1 ,2 ]
Nalban, Nasiruddin [1 ,2 ]
Alavala, Sateesh [1 ]
Rajendran, Vinoth [1 ]
Jerald, Mahesh Kumar [3 ]
Sistla, Ramakrishna [1 ,2 ]
机构
[1] CSIR Indian Inst Chem Technol IICT, Dept Appl Biol, Hyderabad 500007, Andhra Pradesh, India
[2] Acad Sci & Innovat Res AcSIR, CSIR HRDC Campus,Sect 19, Ghaziabad 201002, Uttar Pradesh, India
[3] CSIR Ctr Cellular & Mol Biol CCMB, Anim House Facil, Hyderabad 500007, Andhra Pradesh, India
关键词
Galangin; Ulcerative colitis; Inflammation; NF-kappa B pathway; Dextran sulfate sodium; CISPLATIN-INDUCED NEPHROTOXICITY; NRF2 SIGNALING PATHWAY; OXIDATIVE STRESS; INFLAMMATION; APOPTOSIS; ERK; GLUTATHIONE; MECHANISM; CYTOKINES; COLITIS;
D O I
10.1007/s00011-019-01252-w
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Objective and designInflammatory bowel disease (IBD) is known to cause chronic inflammation in the digestive tract by the immune malfunction. Herein, we demonstrate the protective effect of galangin (GAL), a phytochemical, on LPS-induced inflammation in cultured mouse macrophages (RAW 264.7) and the treatment of DSS-induced ulcerative colitis in Balb/c mice. However, the anti-inflammatory effect of GAL in DSS-exposed experimental colitis has not been investigated.Materials and methodsWe determined the levels of proinflammatory cytokines by ELISA, biochemical analysis using standard protocols and protein expression level of NF-kappa B signaling pathway and activation of Nrf2 gene pathway wereanalyzed by western blot analysis in colitis-induced mice.ResultsOur in vitro studies showed that LPS-stimulated RAW 264.7 cells treated with GAL reduced the levels of nitrites, IL-6, and TNF-alpha in a concentration-dependent manner. The results demonstrated that oral administration of GAL at 20mg/kg (lower dose) and 40mg/kg (higher dose) significantly reduced the severity of colitis and mitigated the clinical signs of both macroscopic and microscopic of the disease. The levels of proinflammatory cytokines (TNF-alpha and IL-6) in colonic tissue and serum were reduced significantly and in GAL+DSS-treated group relative to DSS alone treated group. Increased levels of anti-inflammatory cytokine (IL-10) was detected in colon tissues in GAL+DSS-treated groups relative to DSS alone treated group. We also observed decreased levels of myeloperoxidase (MPO), nitrites and TBARS with increased SOD in colonic tissue of GAL+DSS group. Besides, GAL+DSS-treated animals significantly suppressed protein expressions of p-NF-kappa B and p-Ikk-beta alpha, COX-2, iNOS, Nrf2 and increased HO-1 levels in colon tissues by inhibiting inflammation and oxidative stress.ConclusionOur study highlights the protective effect of galangin as an anti-inflammatory agent against the severe form of colitis in pre-clinical models suggesting its potency for the treatment of IBD in humans.
引用
收藏
页码:691 / 704
页数:14
相关论文
共 50 条
  • [21] Hookworm Products Ameliorate Dextran Sodium Sulfate-induced Colitis in BALB/c Mice
    Lopes Cancado, Guilherme Grossi
    Fiuza, Jacqueline Araujo
    Nogueira de Paiva, Nivia Carolina
    Dhom Lemos, Lucas de Carvalho
    Ricci, Natasha Delaqua
    Gazzinelli-Guimaraes, Pedro Henrique
    Martins, Virgillio Gandra
    Bartholomeu, Daniella Castanheira
    Negrao-Correa, Deborah Aparecida
    Carneiro, Claudia Martins
    Fujiwara, Ricardo Toshio
    INFLAMMATORY BOWEL DISEASES, 2011, 17 (11) : 2275 - 2286
  • [22] VIP Deficiency Results in a Protective Effect in a Murine Model of Dextran Sulfate Sodium (DSS) Induced Colitis
    Vu, John P.
    Million, Mulugeta
    Larauche, Muriel H.
    Waschek, James
    Tache, Yvette
    Pisegna, Joseph R.
    Germano, Patrizia M.
    GASTROENTEROLOGY, 2011, 140 (05) : S479 - S479
  • [23] Chicoric Acid Effectively Mitigated Dextran Sulfate Sodium (DSS)-Induced Colitis in BALB/c Mice by Modulating the Gut Microbiota and Fecal Metabolites
    Yang, Jiani
    Lin, Jie
    Gu, Ting
    Sun, Quancai
    Xu, Weidong
    Peng, Ye
    Aminov, Rustam I.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (02)
  • [24] Protective effect of sugar cane extract against dextran sulfate sodium-induced colonic inflammation in mice
    Wang, Bin
    Li, Yansen
    Mizu, Masami
    Furuta, Toma
    Li, ChunMei
    TISSUE & CELL, 2017, 49 (01): : 8 - 14
  • [25] Protective Effect of Korean Hedyotis diffusa Extract Against Dextran Sulfate Sodium-induced Colitis in Mice
    Kim, Jisu
    Baek, Suji
    Choi, Nan Hee
    Kwon, Seung-Hae
    Lee, So Hui
    Lee, Kang Pa
    INTERNATIONAL JOURNAL OF PHARMACOLOGY, 2020, 16 (04) : 291 - 297
  • [26] Arbutin ameliorated ulcerative colitis of mice induced by dextran sodium sulfate (DSS)
    Zhang, Chuan
    Zhu, Huiping
    Jie, Hui
    Ding, Hengyue
    Sun, Hongwen
    BIOENGINEERED, 2021, 12 (02) : 11707 - 11715
  • [27] Protective effect of Lagerstroemia speciosa against dextran sulfate sodium induced ulcerative colitis in C57BL/6 mice
    Chaudhary, Ghanshyam
    Mahajan, Umesh B.
    Goyal, Sameer N.
    Ojha, Shreesh
    Patil, Chandragouda R.
    Subramanya, Sandeep B.
    AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2017, 9 (04): : 1792 - 1800
  • [28] Protective effect of three glucomannans from different plants against DSS induced colitis in female BALB/c mice
    Zhang, Liu-Jing
    Huang, Xiao-Jun
    Shi, Xiao-Dan
    Chen, Hai-Hong
    Cui, Steve W.
    Nie, Shao-Ping
    FOOD & FUNCTION, 2019, 10 (04) : 1928 - 1939
  • [29] Isaria cicadae Miquel, as an edible fungus, against dextran sulfate sodium-induced ulcerative colitis in BALB/c mice
    Wang, Beiqi
    Li, Xingyang
    Hao, Wenwen
    Yu, Ruilian
    Wu, Hao
    Wang, Qin
    Xu, Li
    Xu, Jinguo
    Wu, Yanling
    JOURNAL OF FUTURE FOODS, 2025, 5 (04): : 398 - 409
  • [30] The effect of olsalazine of chinese generic drugs on ulcerative colitis induced by dextran sulfate sodium salt in BALB/c mice
    Yu, Zheng-Yong
    Xu, Yu-Sheng
    Tang, Miao
    Xin, Wen-Feng
    ACTA CIRURGICA BRASILEIRA, 2023, 38