Structure Activity Relationship of Membrane-Targeting Cationic Ligands on a Silver Nanoparticle Surface in an Antibiotic-Resistant Antibacterial and Antibiofilm Activity Assay

被引:45
作者
Dai, Xiaomei [1 ]
Chen, Xuelei [1 ]
Zhao, Jing [1 ]
Zhao, Yu [1 ]
Guo, Qianqian [1 ]
Zhang, Tianqi [1 ]
Chu, Chunli [2 ]
Zhang, Xinge [1 ]
Li, Chaoxing [1 ]
机构
[1] Nankai Univ, Inst Polymer Chem, Key Lab Funct Polymer Mat, Minist Educ, Tianjin 300071, Peoples R China
[2] Nankai Univ, Coll Environm Sci & Engn, Tianjin 300350, Peoples R China
基金
中国国家自然科学基金;
关键词
antibiotic-resistance; structure-activity relationship; antibacterial mechanism; cationic ligand; biofilms; silver nanoparticles; GRAM-NEGATIVE BACTERIA; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; ANTIMICROBIAL ACTIVITY; BIOFILM DEVELOPMENT; GOLD NANOPARTICLES; CANDIDA-ALBICANS; NYLON-3; POLYMERS; SMALL MOLECULES; AGENTS;
D O I
10.1021/acsami.6b15821
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
To explore The structure-activity relationship of membrane-targeting cationic ligands on a silver nanoparticle surface in an antibiotic-resistant antibacterial and antibiofilm activity assay, a series of functionalized silver nanocomposites were synthesized. Tuning the structural configuration, molecular weight, and side-chain length of the cationic ligands on the nanoparticle surface provided silver nanocomposites with effective antibacterial activity, against both antibiotic resistant Gram-negative and Gram-positive bacteria, including bacterial biofilins. These silver nanocomposites did not trigger hemolytic activity. Significantly; the bacteria did not develop resistance to the obtained nanocomposites even after 30 generations. A. study of the antibacterial mechanism confirmed that these nanocomposites Could irreversibly disrupt the membrane,structure of bacteria and effectively inhibit-intracellular enzyme activity, ultimately leading to bacterial, death. The silver nanocomposites (64 mu g/mL) could eradicate. 80% of an established antibiotic-resistant bacterial biofilm. The strong structure activity relationship toward antibacterial and antibiofilm activity suggests that variations in the conformational prOperty of the functional ligand could be valuable in the discovery of new nano antibacterial agents for treating pathogenic bacterial infections.
引用
收藏
页码:13837 / 13848
页数:12
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