Beyond homing: Competition between intron endonucleases confers a selective advantage on flanking genetic markers

被引:79
作者
GoodrichBlair, H [1 ]
Shub, DA [1 ]
机构
[1] SUNY ALBANY, CTR GENET MOLEC, DEPT BIOL SCI, ALBANY, NY 12222 USA
关键词
D O I
10.1016/S0092-8674(00)80976-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The closely related B. subtilis bacteriophages SPO1 and SP82 have similar introns inserted into a conserved domain of their DNA polymerase genes. These introns encode endonucleases with unique properties. Other intron-encoded ''homing'' endonucleases cleave both strands of intronless DNA; subsequent repair results in unidirectional gene conversion to the intron-containing allele. In contrast, the enzymes described here cleave one strand on both intron-containing and intronless targets at different distances from their common intron insertion site. Most surprisingly, each enzyme prefers DNA of the heterologous phage. The SP82-encoded endonuclease is responsible for exclusion of the SPO1 intron and flanking genetic markers from the progeny of mixed infections, a novel selective advantage imparted by an intron to the genome in which it resides.
引用
收藏
页码:211 / 221
页数:11
相关论文
共 40 条
  • [1] SELECTION OF A REMOTE CLEAVAGE SITE BY I-TEVI, THE TD INTRON-ENCODED ENDONUCLEASE
    BRYK, M
    BELISLE, M
    MUELLER, JE
    BELFORT, M
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1995, 247 (02) : 197 - 210
  • [2] CULL M, 1990, METHOD ENZYMOL, V182, P147
  • [3] CLONING AND MAPPING OF THE SPO1 GENOME
    CURRAN, JF
    STEWART, CR
    [J]. VIROLOGY, 1985, 142 (01) : 78 - 97
  • [4] A SITE-SPECIFIC ENDONUCLEASE ENCODED BY A TYPICAL ARCHAEAL INTRON
    DALGAARD, JZ
    GARRETT, RA
    BELFORT, M
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) : 5414 - 5417
  • [5] PROTEIN SPLICING IN THE MATURATION OF MYCOBACTERIUM-TUBERCULOSIS RECA PROTEIN - A MECHANISM FOR TOLERATING A NOVEL CLASS OF INTERVENING SEQUENCE
    DAVIS, EO
    JENNER, PJ
    BROOKS, PC
    COLSTON, MJ
    SEDGWICK, SG
    [J]. CELL, 1992, 71 (02) : 201 - 210
  • [7] MOBILE INTRONS - DEFINITION OF TERMS AND RECOMMENDED NOMENCLATURE
    DUJON, B
    BELFORT, M
    BUTOW, RA
    JACQ, C
    LEMIEUX, C
    PERLMAN, PS
    VOGT, VM
    [J]. GENE, 1989, 82 (01) : 115 - 118
  • [8] THE PHAGE-T4 NRDB INTRON - A DELETION MUTANT OF A VERSION FOUND IN THE WILD
    EDDY, SR
    GOLD, L
    [J]. GENES & DEVELOPMENT, 1991, 5 (06) : 1032 - 1041
  • [9] HOMING OF A DNA ENDONUCLEASE GENE BY MEIOTIC GENE CONVERSION IN SACCHAROMYCES-CEREVISIAE
    GIMBLE, FS
    THORNER, J
    [J]. NATURE, 1992, 357 (6376) : 301 - 306
  • [10] INITIATION AND TERMINATION MUTANTS OF BACILLUS-SUBTILIS BACTERIOPHAGE-SPO1
    GLASSBERG, J
    FRANCK, M
    STEWART, CR
    [J]. JOURNAL OF VIROLOGY, 1977, 21 (01) : 147 - 152