共 52 条
Brca2 is involved in meiosis in Arabidopsis thaliana as suggested by its interaction with Dmc1
被引:132
作者:

Siaud, N
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Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France

Dray, E
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Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France

Gy, I
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Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France

Gérard, E
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机构:
Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France

Takvorian, N
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Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France

Doutriaux, MP
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Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France
机构:
[1] Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France
关键词:
Arabidopsis;
Brca2;
Dmc1;
meiosis;
Rad51;
D O I:
10.1038/sj.emboj.7600146
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Two BRCA2-like sequences are present in the Arabidopsis genome. Both genes are expressed in flower buds and encode nearly identical proteins, which contain four BRC motifs. In a yeast two-hybrid assay, the Arabidopsis Brca2 proteins interact with Rad51. and Dmc1. RNAi constructs aimed at silencing the BRCA2 genes at meiosis triggered a reproducible sterility phenotype, which was associated with dramatic meiosis alterations. We obtained the same phenotype upon introduction of RNAi constructs aimed at silencing the RAD51 gene at meiosis in dmc1 mutant plants. The meiotic figures we observed strongly suggest that homologous recombination is highly disturbed in these meiotic cells, leaving aberrant recombination events to repair the meiotic double-strand breaks. The 'brca2' meiotic phenotype was eliminated in spoil mutant plants. Our experiments point to an essential role of Brca2 at meiosis in Arabidopsis. We also propose a role for Rad51 in the dmc1 context.
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页码:1392 / 1401
页数:10
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