Brca2 is involved in meiosis in Arabidopsis thaliana as suggested by its interaction with Dmc1

被引:132
作者
Siaud, N [1 ]
Dray, E [1 ]
Gy, I [1 ]
Gérard, E [1 ]
Takvorian, N [1 ]
Doutriaux, MP [1 ]
机构
[1] Univ Paris 11, Inst Biotechnol Plantes, CNRS, UMR 8618, F-91405 Orsay, France
关键词
Arabidopsis; Brca2; Dmc1; meiosis; Rad51;
D O I
10.1038/sj.emboj.7600146
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two BRCA2-like sequences are present in the Arabidopsis genome. Both genes are expressed in flower buds and encode nearly identical proteins, which contain four BRC motifs. In a yeast two-hybrid assay, the Arabidopsis Brca2 proteins interact with Rad51. and Dmc1. RNAi constructs aimed at silencing the BRCA2 genes at meiosis triggered a reproducible sterility phenotype, which was associated with dramatic meiosis alterations. We obtained the same phenotype upon introduction of RNAi constructs aimed at silencing the RAD51 gene at meiosis in dmc1 mutant plants. The meiotic figures we observed strongly suggest that homologous recombination is highly disturbed in these meiotic cells, leaving aberrant recombination events to repair the meiotic double-strand breaks. The 'brca2' meiotic phenotype was eliminated in spoil mutant plants. Our experiments point to an essential role of Brca2 at meiosis in Arabidopsis. We also propose a role for Rad51 in the dmc1 context.
引用
收藏
页码:1392 / 1401
页数:10
相关论文
共 52 条
[1]   The EMB 506 gene encodes a novel ankyrin repeat containing protein that is essential for the normal development of Arabidopsis embryos [J].
Albert, S ;
Després, B ;
Guilleminot, J ;
Bechtold, N ;
Pelletier, G ;
Delseny, M ;
Devic, M .
PLANT JOURNAL, 1999, 17 (02) :169-179
[2]   An atypical topoisomerase II from archaea with implications for meiotic recombination [J].
Bergerat, A ;
deMassy, B ;
Gadelle, D ;
Varoutas, PC ;
Nicolas, A ;
Forterre, P .
NATURE, 1997, 386 (6623) :414-417
[3]   The BRC repeats are conserved in mammalian BRCA2 proteins [J].
Bignell, G ;
Micklem, G ;
Stratton, MR ;
Ashworth, A ;
Wooster, R .
HUMAN MOLECULAR GENETICS, 1997, 6 (01) :53-58
[4]   High copy number suppression of the meiotic arrest caused by a dmc1 mutation:: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway [J].
Bishop, DK ;
Nikolski, Y ;
Oshiro, J ;
Chon, J ;
Shinohara, M ;
Chen, X .
GENES TO CELLS, 1999, 4 (08) :425-443
[5]   The Arabidopsis homologue of Xrcc3 plays an essential role in meiosis [J].
Bleuyard, JY ;
White, CI .
EMBO JOURNAL, 2004, 23 (02) :439-449
[6]   Internal repeats in the BRCA2 protein sequence [J].
Bork, P ;
Blomberg, N ;
Nilges, M .
NATURE GENETICS, 1996, 13 (01) :22-23
[7]   Expression profiling of the whole Arabidopsis Shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction [J].
Charrier, B ;
Champion, A ;
Henry, Y ;
Kreis, M .
PLANT PHYSIOLOGY, 2002, 130 (02) :577-590
[8]   Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells [J].
Chen, JJ ;
Silver, DP ;
Walpita, D ;
Cantor, SB ;
Gazdar, AF ;
Tomlinson, G ;
Couch, FJ ;
Weber, BL ;
Ashley, T ;
Livingston, DM ;
Scully, R .
MOLECULAR CELL, 1998, 2 (03) :317-328
[9]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[10]   Tumorigenesis and a DNA repair defect in mice with a truncating Brca2 mutation [J].
Connor, F ;
Bertwistle, D ;
Mee, PJ ;
Ross, GM ;
Swift, S ;
Grigorieva, E ;
Tybulewicz, VLJ ;
Ashworth, A .
NATURE GENETICS, 1997, 17 (04) :423-430