Control of pre-BCR signaling by Pax5-dependent activation of the BLNK gene

被引：132

作者：

Schebesta, M ^{[1
]}

Pfeffer, PL ^{[1
]}

Busslinger, M ^{[1
]}

机构：

[1] Vienna Bioctr, Res Inst Mol Pathol, A-1030 Vienna, Austria

来源：

IMMUNITY | 2002年 / 17卷 / 04期

关键词：

D O I：

10.1016/S1074-7613(02)00418-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The developmental progression from pro-B to pre-B cells is controlled by pre-B cell receptor (pre-BCR) signaling which depends on BLNK (SLP-65) for coupling the Syk kinase to its downstream effector pathways. Here we identified BLNK as a direct target of the transcription factor Pax5 (BSAP). Restoration of BLNK expression in Igmu transgenic Pax5(-/-) pro-B cells resulted in constitutive pre-BCR signaling and increased cell proliferation without inducing progression to the pre-B cell stage. Igmu(+) Pax5(-/-) pro-B cells expressing a BLNK-estrogen receptor fusion protein initiated signaling immediately upon hormone addition, which facilitated analysis of pre-BCR-induced gene expression changes. The pre-BCR was shown to execute its checkpoint function by regulating genes involved in cell proliferation, intracellular signaling, growth factor responsiveness, and V(D)J recombination.

引用

页码：473 / 485

页数：13

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