A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets

被引:82
作者
Lin, Tianran [1 ]
Zhong, Liangshuang [1 ]
Chen, Huan [1 ]
Li, Zhihong [1 ]
Song, Zhiping [1 ]
Guo, Liangqia [1 ]
Fu, Fengfu [1 ]
机构
[1] Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China
关键词
Enzyme mimic; Tetramethylbenzidine; Human serum; Colorimetric method; Hydrogen peroxide; Cholesterol oxidase; FLUORESCENT DETECTION; HYDROGEN-PEROXIDE; BIOSENSOR; PERFORMANCE; GLUCOSE; SERUM; CHROMATOGRAPHY; OXIDASE; SENSOR;
D O I
10.1007/s00604-017-2147-x
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The authors describe a colorimetric method for the determination of cholesterol in human serum. Cholesterol is enzymatically oxidized by oxygen in the presence of cholesterol oxidase (ChOx) to produce 4-cholestene-3-one and hydrogen peroxide (H2O2). Due to the peroxidase-like activity of MoS2 nanosheets, H2O2 can oxidize the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) to give a blue product. The increase in the absorbance of the acidic oxTMB solution at 450 nm is used for quantification of cholesterol. Under the optimal conditions, the increase in absorbance is proportional to the concentration of cholesterol in the range from 2 to 200 mu molai...L-1, and the limit of detection is 0.76 mu molai...L-1. The color change from pale yellow to blue color can also be detected visually for cholesterol concentrations as low as 20 mu molai...L-1. Such an enzyme mimetic-based assay possesses advantages over enzyme-based assays in terms of costs, stability against denaturation, and protease digestion. The assay was applied to the determination of free cholesterol and of total cholesterol (after hydrolysis using an esterase) in spiked human serum, and it gave satisfactory recoveries.
引用
收藏
页码:1233 / 1237
页数:5
相关论文
共 30 条
  • [1] High-performance cholesterol sensor based on the solution-gated field effect transistor fabricated with ZnO nanorods
    Ahmad, Rafiq
    Tripathy, Nirmalya
    Hahn, Yoon-Bong
    [J]. BIOSENSORS & BIOELECTRONICS, 2013, 45 : 281 - 286
  • [2] Impurity-induced peroxidase mimicry of nanoclay and its potential for the spectrophotometric determination of cholesterol
    Aneesh, K.
    Vusa, Chiranjeevi Srinivasa Rao
    Berchmans, Sheela
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (22) : 6213 - 6221
  • [3] Poly-(3-hexylthiophene) self-assembled monolayer based cholesterol biosensor using surface plasmon resonance technique
    Arya, Sunil K.
    Solanki, Pratima R.
    Singh, S. P.
    Kaneto, K.
    Pandey, Manoj K.
    Datta, Monika
    Malhotra, Bansi D.
    [J]. BIOSENSORS & BIOELECTRONICS, 2007, 22 (11) : 2516 - 2524
  • [4] Gold Nanocluster-Assisted Fluorescent Detection for Hydrogen Peroxide and Cholesterol Based on the Inner Filter Effect of Gold Nanoparticles
    Chang, Heng-Chia
    Ho, Ja-an Annie
    [J]. ANALYTICAL CHEMISTRY, 2015, 87 (20) : 10362 - 10367
  • [5] Chemically Exfoliated MoS2 as Near-Infrared Photothermal Agents
    Chou, Stanley S.
    Kaehr, Bryan
    Kim, Jaemyung
    Foley, Brian M.
    De, Mrinmoy
    Hopkins, Patrick E.
    Huang, Jiaxing
    Brinker, C. Jeffrey
    Dravid, Vinayak P.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (15) : 4160 - 4164
  • [6] Performance-enhanced cholesterol biosensor based on biocomposite system: Layered double hydroxides-chitosan
    Ding, Shou-Nian
    Shan, Dan
    Zhang, Tao
    Dou, Yan-Zhi
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2011, 659 (01) : 1 - 5
  • [7] Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor
    Gomathi, Palanisamy
    Ragupathy, Dhanusuraman
    Choi, Jin Hyun
    Yeum, Jeong Hyun
    Lee, Soo Chool
    Kim, Jae Chang
    Lee, Sang Hak
    Do Ghim, Han
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2011, 153 (01) : 44 - 49
  • [8] Simultaneous determination of serum lathosterol and cholesterol by semi-micro high-performance liquid chromatography with electrochemical detection
    Hojo, Kazuhiro
    Hakamata, Hideki
    Kusu, Fumiyo
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (11-12): : 751 - 755
  • [9] A dual enzymatic-biosensor for simultaneous determination of glucose and cholesterol in serum and peritoneal macrophages of diabetic mice: Evaluation of the diabetes-accelerated atherosclerosis risk
    Huang, Qilin
    An, Yarui
    Tang, Linlin
    Jiang, Xiaoli
    Chen, Hua
    Bi, Wenji
    Wang, Zhongchuan
    Zhang, Wen
    [J]. ANALYTICA CHIMICA ACTA, 2011, 707 (01) : 135 - 141
  • [10] Metal dichalcogenide nanosheets: preparation, properties and applications
    Huang, Xiao
    Zeng, Zhiyuan
    Zhang, Hua
    [J]. CHEMICAL SOCIETY REVIEWS, 2013, 42 (05) : 1934 - 1946