Genome-Wide Identification and Capsaicinoid Biosynthesis-Related Expression Analysis of the R2R3-MYB Gene Family in Capsicum annuum L.

被引:38
作者
Wang, Jin [1 ,2 ]
Liu, Yi [3 ]
Tang, Bingqian [3 ]
Dai, Xiongze [2 ,4 ]
Xie, Lingling [5 ]
Liu, Feng [4 ,5 ]
Zou, Xuexiao [2 ,4 ]
机构
[1] Nanjing Agr Univ, Coll Hort, Nanjing, Peoples R China
[2] Minist Educ, Engn Res Ctr Hort Crop Germplasm Creat & New Vari, Changsha, Peoples R China
[3] Hunan Univ, Longping Branch, Grad Sch, Changsha, Peoples R China
[4] Hunan Agr Univ, Coll Hort, Changsha, Peoples R China
[5] Hunan Vegetable Res Inst, Changsha, Peoples R China
基金
中国国家自然科学基金;
关键词
Capsicum; capsaicinoid biosynthesis; expression analysis; co-expression; TRANSCRIPTION FACTORS; PROVIDES INSIGHTS; PUNGENCY; SEQUENCE; EVOLUTION; PEPPERS; SERVER; MODEL;
D O I
10.3389/fgene.2020.598183
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Capsaicinoids are naturally specialized metabolites in pepper and are the main reason that Capsicum fruits have a pungent smell. During the synthesis of capsaicin, MYB transcription factors play key regulatory roles. In particular, R2R3-MYB subfamily genes are the most important members of the MYB family and are critical candidate factors in capsaicinoid biosynthesis. The 108 R2R3-MYB genes in pepper were identified in this study and all are shown to have two highly conserved MYB binding domains. Phylogenetic and structural analyses clustered CaR2R3-MYB genes into seven groups. Interspecies collinearity analysis found that the R2R3-MYB family contains 16 duplicated gene pairs and the highest gene density is on chromosome 00 and 03. The expression levels of CaR2R3-MYB differentially expressed genes (DEGs) and capsaicinoid-biosynthetic genes (CBGs) in fruit development stages were obtained via RNA-seq and quantitative polymerase chain reaction (qRT-PCR). Co-expression analyses reveal that highly expressed CaR2R3-MYB genes are co-expressed with CBGs during early stages of pericarp and placenta development processes. It is speculated that six candidate CaR2R3-MYB genes are involved in regulating the synthesis of capsaicin and dihydrocapsaicin. This study is the first systematic analysis of the CaR2R3-MYB gene family and provided references for studying their molecular functions. At the same time, these results also laid the foundation for further research on the capsaicin characteristics of CaR2R3-MYB genes in pepper.
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页数:12
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