Effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling

被引：42
作者：
Horak, Daniel [1 ,2 ]
Babic, Michal [1 ,2 ]
Jendelova, Pavla [2 ,3 ]
Herynek, Vit [4 ]
Trchova, Miroslava [1 ]
Likavcanova, Katarina [3 ]
Kapcalova, Miroslava [3 ]
Hajek, Milan [2 ,4 ]
Sykova, Eva [2 ,3 ]
机构：
[1] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague 6, Czech Republic
[2] Charles Univ Prague, Ctr Cell Therapy & Tissue Repair, Prague 15006 5, Czech Republic
[3] Acad Sci Czech Republ, Inst Expt Med, Prague 14220 4, Czech Republic
[4] Inst Clin & Expt Med, Prague 14021 4, Czech Republic
来源：
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2009年 / 321卷 / 10期
关键词：
Magnetic nanoparticles; Maghemite; MRI; Stem cells; Biocompatibility; Contrast agent; IRON-OXIDE NANOPARTICLES; IN-VIVO TRACKING; CONTRAST AGENTS; TRANSPLANTATION; RECOVERY; TAT;
D O I：
10.1016/j.jmmm.2009.02.082
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Maghemite nanoparticles with various coatings were prepared by the coprecipitation method and characterized by transmission electron microscopy, dynamic light scattering and IR in terms of morphology, size, polydispersity and surface coating. The labeling efficiency and the viability of both rat and human mesenchymal stem cells labeled with Endorem (R), poly(L-lysine) (PLL)-modified Endorem (R), uncoated gamma-Fe2O3, D-mannose-, PLL- or poly(N,N-dimethylacrylamide) (PDMAAm)-coated gamma-Fe2O3 nanoparticles were compared. Coated gamma-Fe2O3 nanoparticles labeled cells better than did Endorem (R). High relaxation rates and in vitro magnetic resonance imaging of cells labeled with coated nanoparticles showed clearly visible contrast compared with unlabeled cells or cells labeled with Endorem (R). (c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：1539 / 1547
页数：9
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