Sucrose hydrolysis by invertase using a membrane reactor: effect of membrane cut-off on enzyme performance

被引:2
作者
Di Addezio, Francesco [1 ]
Yoriyaz, Ester Junko [2 ]
Cantarella, Maria [1 ]
Vitolo, Michele [2 ]
机构
[1] Univ Aquila, Dept Chem Engn, I-67100 Laquila, Italy
[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Invertase; Membrane Reactor; Sucrose/hydrolysis;
D O I
10.1590/S1984-82502014000200004
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Sucrose hydrolysis by invertase [EC.3.2.1.26] produces inverted sugar syrup, an ingredient mainly used in the food industry. To properly catalyze hydrolysis, the enzyme should be reused after this reaction. It is advisable to maintain constant activity over a considerable period. Thus, sucrose hydrolysis was performed in a membrane bioreactor - a continuously stirred tank reactor coupled with an ultrafiltration membrane (UFM) which provides good diffusion and high activity per unit volume. Molecular weight cut-off for soluble invertase UFMs was up to 100kDa. This study focused on the role of UFM invertase cut-off as it is the main element in the process. We demonstrated that both the cut-off and chemical nature of the UFM affected specific invertase activity.
引用
收藏
页码:257 / 259
页数:3
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