Diagnosis of tuberculosis by nanoparticle-based immuno-PCR assay based on mycobacterial MPT64 and CFP-10 detection

被引：36

作者：

Dahiya, Bhawna ^{[1
]}

Prasad, Tulika ^{[2
]}

Singh, Vishwajeet ^{[3
]}

Khan, Anish ^{[1
]}

Kamra, Ekta ^{[1
]}

Mor, Preeti ^{[1
]}

Yadav, Aparna ^{[4
]}

Gupta, Krishna B. ^{[5
]}

Mehta, Promod K. ^{[1
]}

机构：

[1] Maharshi Dayanand Univ MDU, Ctr Biotechnol, Rohtak 124001, Haryana, India

[2] Jawaharlal Nehru Univ JNU, Special Ctr Nano Sci & Adv Instrumentat Res & Fac, New Delhi 110067, India

[3] All India Inst Med Sci, Dept Biostat, New Delhi 110029, India

[4] Univ Hlth Sci UHS, Dept Microbiol, Rohtak 124001, Haryana, India

[5] UHS, Dept TB & Resp Med, Rohtak 124001, Haryana, India

来源：

NANOMEDICINE | 2020年 / 15卷 / 26期

关键词：

diagnosis; functionalized gold nanoparticles; GeneXpert; gold nanoparticles; M; tuberculosis; magnetic bead-coupled gold nanoparticle-based immuno-PCR; magnetic beads; magneto-ELISA; MPT64+CFP-10; PROBE-BASED ASSAY; BIO-BARCODE; SENSITIVE DETECTION; ANTIGEN; 85B; PULMONARY; ESAT-6; SERODIAGNOSIS; AMPLIFICATION;

D O I：

10.2217/nnm-2020-0258

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim:To improve the diagnostic accuracy of immuno-PCR (I-PCR) in tuberculosis (TB) patients by using functionalized gold nanoparticles (AuNPs) coupled with detection antibodies and oligonucleotides, and magnetic beads (MBs) conjugated with capture antibodies in the liquid phase.Materials & methods:MB-coupled AuNP-based I-PCR (MB-AuNP-I-PCR) assay was designed to detect a cocktail ofMycobacterium tuberculosisMPT64 and CFP-10 proteins in bodily fluids of TB patients.Results:The sensitivities of 89.3 (n = 94) and 78.1% (n = 73) were observed in pulmonary TB and extrapulmonary TB patients, respectively, with specificities of 97.9-98.3%. Notably, the sensitivities attained by MB-AuNP-I-PCR in smear-negative pulmonary TB and extrapulmonary TB patients were significantly higher (p < 0.05-0.001) than Magneto-ELISA and GeneXpert assay.Conclusion:The improved technology, as well as enhanced diagnostic accuracy of MB-AuNP-I-PCR, may lead to development of an attractive diagnostic kit.

引用

页码：2609 / 2624

页数：16

共 39 条

[1] Immunomagnetic quantitative immuno-PCR for detection of less than one HIV-1 virion [J].

Barletta, Janet ;

Bartolome, Amelita ;

Constantine, Niel T. .

JOURNAL OF VIROLOGICAL METHODS, 2009, 157 (02) :122-132

[2] CD4 quantification based on magneto ELISA for AIDS diagnosis in low resource settings [J].

Carinelli, S. ;

Xufre, C. ;

Alegret, S. ;

Marti, M. ;

Pividori, M. I. .

TALANTA, 2016, 160 :36-45

[3] Semisynthetic biogenic magnetosome nanoparticles for the detection of proteins and nucleic acids [J].

Ceyhan, Buelent ;

Alhorn, Petra ;

Lang, Claus ;

Schueler, Dirk ;

Niemeyer, Christof M. .

SMALL, 2006, 2 (11) :1251-1255

[4] Detection of mycobacterial CFP-10 (Rv3874) protein in tuberculosis patients by gold nanoparticle-based real-time immuno-PCR [J].

Dahiya, Bhawna ;

Sharma, Suman ;

Khan, Anish ;

Kamra, Ekta ;

Mor, Preeti ;

Sheoran, Abhishek ;

Sreenivas, Vishnubhatla ;

Varma-Basil, Mandira ;

Gupta, Krishna B. ;

Gupta, Mahesh C. ;

Chaudhary, Dhruva ;

Mehta, Promod K. .

FUTURE MICROBIOLOGY, 2020, 15 (08) :601-612

[5]

Dahiya B, 2019, PATHOG DIS, V77, DOI 10.1093/femspd/ftz049

[6] Detection of potential biomarkers associated with outrageous diseases and environmental pollutants by nanoparticle-based immuno-PCR assays [J].

Dahiya, Bhawna ;

Mehta, Promod K. .

ANALYTICAL BIOCHEMISTRY, 2019, 587

[7] Xpert MTB/RIF assay for the diagnosis of pulmonary tuberculosis in children: a systematic review and meta-analysis [J].

Detjen, Anne K. ;

DiNardo, Andrew R. ;

Leyden, Jacinta ;

Steingart, Karen R. ;

Menzies, Dick ;

Schiller, Ian ;

Dendukuri, Nandini ;

Mandalakas, Anna M. .

LANCET RESPIRATORY MEDICINE, 2015, 3 (06) :451-461

[8] World Health Organization cardiovascular disease risk charts: revised models to estimate risk in 21 global regions [J].

Di Angelantonio, Emanuele ;

Kaptoge, Stephen ;

Pennells, Lisa ;

De Bacquer, Dirk ;

Cooney, Marie Therese ;

Kavousi, Maryam ;

Stevens, Gretchen ;

Riley, Leanne ;

Savin, Stefan ;

Altay, Servet ;

Amouyel, Philippe ;

Assmann, Gerd ;

Bell, Steven ;

Ben-Shlomo, Yoav ;

Berkman, Lisa ;

Beulens, Joline W. ;

Bjorkelund, Cecilia ;

Blaha, Michael J. ;

Blazer, Dan G. ;

Bolton, Thomas ;

Bonita, Ruth ;

Brenner, Beaglehole Hermann ;

Brunner, Eric J. ;

Casiglia, Edoardo ;

Chamnan, Parinya ;

Choi, Yeun-Hyang ;

Chowdhury, Rajiv ;

Coady, Sean ;

Crespo, Carlos J. ;

Cushman, Mary ;

Dagenais, Gilles R. ;

D'Agostino, Ralph B. ;

Daimon, Makoto ;

Davidson, Karina W. ;

Engstrom, Gunnar ;

Fang, Xianghua ;

Ford, Ian ;

Gallacher, John ;

Gansevoort, Ron T. ;

Gaziano, Thomas Andrew ;

Giampaoli, Simona ;

Grandits, Greg ;

Grimsgaard, Sameline ;

Grobbee, Diederick E. ;

Gudnason, Vilmundur ;

Guo, Qi ;

Humphries, Steve ;

Iso, Hiroyasu ;

Jukema, J. Wouter ;

Kauhanen, Jussi .

LANCET GLOBAL HEALTH, 2019, 7 (10) :E1332-E1345

[9] Two types of nanoparticle-based bio-barcode amplification assays to detect HIV-1 p24 antigen [J].

Dong, Huahuang ;

Liu, Jianli ;

Zhu, Hong ;

Ou, Chin-Yih ;

Xing, Wenge ;

Qiu, Maofeng ;

Zhang, Guiyun ;

Xiao, Yao ;

Yao, Jun ;

Pan, Pinliang ;

Jiang, Yan .

VIROLOGY JOURNAL, 2012, 9

[10] Detection of HIV-1 p24 Gag in plasma by a nanoparticle-based bio-barcode-amplification method [J].

Kim, Eun-Young ;

Stanton, Jennifer ;

Korber, Bette T. M. ;

Krebs, Kendall ;

Bogdan, Derek ;

Kunstman, Kevin ;

Wu, Samuel ;

Phair, John P. ;

Mirkin, Chad A. ;

Wolinsky, Steven M. .

NANOMEDICINE, 2008, 3 (03) :293-303

← 1 2 3 4 →