Curcumin inhibits growth potential by G1 cell cycle arrest and induces apoptosis in p53-mutated COLO 320DM human colon adenocarcinoma cells

被引：38

作者：

Dasiram, Jade Dhananjay ^{[1
]}

Ganesan, Ramamoorthi ^{[1
]}

Kannan, Janani ^{[1
]}

Kotteeswaran, Venkatesan ^{[1
]}

Sivalingam, Nageswaran ^{[1
]}

机构：

[1] SRM Univ, Sch Bioengn, Dept Biotechnol, Kattankulathur 603203, Tamil Nadu, India

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2017年 / 86卷

关键词：

Colon adenocarcinoma; Dukes' type C metastatic stage; Curcumin; Tumor suppressor protein p53; Apoptosis; Cell cycle; CANCER CELLS; COLORECTAL-CANCER; ADJUVANT CHEMOTHERAPY; MOLECULAR TARGETS; CARCINOGENESIS; PATHOGENESIS; DERIVATIVES; MUTATION; THERAPY; EXTRACT;

D O I：

10.1016/j.biopha.2016.12.034

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Curcumin, a natural polyphenolic compound and it is isolated from the rhizome of Curcuma longa, have been reported to possess anticancer effect against stage I and II colon cancer. However, the effect of curcumin on colon cancer at Dukes' type C metastatic stage III remains still unclear. In the present study, we have investigated the anticancer effects of curcumin on p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage. The cellular viability and proliferation were assessed by trypan blue exclusion assay and MTT assay, respectively. The cytotoxicity effect was examined by lactate dehydrogenase (LDH) cytotoxicity assay. Apoptosis was analyzed by DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis. Cell cycle distribution was performed by flow cytometry analysis. Here we have observed that curcumin treatment significantly inhibited the cellular viability and proliferation potential of p53 mutated COLO 320DM cells in a dose-and time-dependent manner. In addition, curcumin treatment showed no cytotoxic effects to the COLO 320DM cells. DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis revealed that curcumin treatment induced apoptosis in COLO 320DM cells. Furthermore, curcumin caused cell cycle arrest at the G1 phase, decreased the cell population in the S phase and induced apoptosis in COLO 320DM colon adenocarcinoma cells. Together, these data suggest that curcumin exerts anticancer effects and induces apoptosis in p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage. (C) 2016 Elsevier Masson SAS. All rights reserved.

引用

页码：373 / 380

页数：8

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