Rapid identification of linezolid resistance in Enterococcus spp. based on high-resolution melting analysis

被引：9

作者：

Alonso, Maria ^{[1
,2
]}

Marin, Mercedes ^{[1
,2
]}

Iglesias, Cristina ^{[1
,2
]}

Cercenado, Emilia ^{[1
,2
]}

Bouza, Emilio ^{[1
,2
,3
]}

Garcia de Viedma, Dario ^{[1
,2
,4
]}

机构：

[1] Univ Gregorio Maranon, Gen Hosp, Inst Invest Sanitaria Gregorio Maranon, Serv Microbiol Clin & Enfermedades Infecciosas, Madrid 28007, Spain

[2] CIBER Enfermedades Resp CIBERES, CD06 06 0058, Madrid, Spain

[3] Univ Complutense Madrid, Fac Med, E-28040 Madrid, Spain

[4] UPM, UCM, CEI Campus Moncloa, Madrid, Spain

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 98卷

关键词：

G2576T; HRM; Enterococcus; 23S rRNA copies; REAL-TIME PCR; STAPHYLOCOCCUS-EPIDERMIDIS; G2576T MUTATION; RIBOSOMAL-RNA; GENE DOSAGE; FAECALIS; FAECIUM;

D O I：

10.1016/j.mimet.2013.12.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymorphism G2576T in the 23S rRNA gene is the mutation most frequently associated with linezolid resistance in Enterococcus spp. isolates. We designed a high-resolution melting procedure to detect G2576T which succeeded in identifying linezolid resistant isolates and classified them according to the number of 23S rRNA copies mutated. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：41 / 43

页数：3

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