Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells

被引：4
|
作者：
Wu, Xiaoping [1 ]
Pang, Qiujie [1 ]
Zhi, Fuxiang [1 ]
Mao, Xiaohong [1 ]
Hu, Yingbin [2 ]
机构：
[1] 1 Peoples Hosp Pinghu, Dept Gen Surg, Pinghu, Peoples R China
[2] Shandong Binzhou Med Univ Hosp, Dept Gastroenterol, 661 Huanghe 2nd Rd, Binzhou City 256603, Shandong, Peoples R China
来源：
TISSUE & CELL | 2022年 / 76卷
关键词：
Colorectal Cancer; Long non-coding RNA; ACTA2 antisense RNA 1; Proliferation; METASTASIS;
D O I：
10.1016/j.tice.2022.101769
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
The role of long non-coding RNA ACTA2 antisense RNA 1 (LncRNA ACTA2-AS1) in colorectal cancer (CRC) was awaited to be elucidated. Clinical specimen and data on ACTA2-AS1 expression in colon adenocarcinoma (COAD) were collected, followed by in situ hybridization. Transfected CRC cell viability, proliferation, migration, and invasion were determined with Cell Counting Kit-8, colony formation, Scratch, and Transwell assays, respectively. Relative expressions of ACTA2-AS1, PCNA, Bcl-2, MMP-2 and MMP-9 were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. ACTA2-AS1 expression was downregulated in CRC. Overexpressed ACTA2-AS1 repressed the cell viability, proliferation, migration and invasion, increased cleaved caspase-3 level yet decreased PCNA, Bcl-2, MMP-2 and MMP-9 levels. ACTA2-AS1 silencing, however, did oppositely. Collectively, ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of CRC cells to effectively suppress the progression of CRC.
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页数：11
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