Fast and non-destructive determination of histamine in tuna fish by ATR-FTIR spectroscopy combined with PLS calibration method

Herein, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy as a non-destructive, rapid and robust method (Mid, 600-4000 cm(-1)) has been examined for the determination of Histamine in tuna fish. For this purpose, a partial least squares regression (PLS-R) with two wavelength selection methods (IPLS and GA) was applied as a multivariate calibration method and the calibration model was developed by cross validation method. After the evaluation of the model on the unknown sample, the results showed that root mean square error of cross validation (RMSECV = 1.10) and root mean square error of prediction (RMSEP = 1.65) were smaller for GA-PLS and correlation coefficient (R-pred(2) = 0.99) were higher than the i-PLS (RMSECV = 3.16, RMSEP = 3.80,R-pred(2) = 0.96). The lower quantification limit was obtained, 4.68 ppm for GA-PLS in a concentration range from 5 to 100 ppm. Hence the results illustrate that ATR/FTIR spectroscopy with PLS can be a feasible method for quick and reliable quantitative analysis of Histamine in the fish products industry.