Measurement of Thermal Conductivities of Two Cryoprotective Agent Solutions for Vitreous Cryopreservation of Organs at the Temperature Range of 77 K-300 K Using a Thermal Sensor Made of Microscale Enamel Copper Wire

被引：2

作者：

Li, Yufang ^{[1
]}

Zhao, Gang ^{[1
,2
]}

Hossain, S. M. Chapal ^{[1
]}

Panhwar, Fazil ^{[1
]}

Sun, Wenyu ^{[3
]}

Kong, Fei ^{[3
]}

Zang, Chuanbao ^{[3
]}

Jiang, Zhendong ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Elect Sci & Technol, Rd JinZhai 96, Hefei 230027, Peoples R China

[2] Anhui Prov Engn Technol Res Ctr Biopreservat & Ar, Hefei, Peoples R China

[3] Yinfeng Cryomed Technol Co Ltd, Jinan, Peoples R China

来源：

BIOPRESERVATION AND BIOBANKING | 2017年 / 15卷 / 03期

基金：

中国国家自然科学基金;

关键词：

vitrification; thermal conductivity; hot probe; the least square method; the Monte Carlo inversion method; LIVING CELLS; VITRIFICATION; LIQUIDS; PROBE; OOCYTES; EMBRYOS; WATER;

D O I：

10.1089/bio.2016.0047

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Biobanking of organs by cryopreservation is an enabling technology for organ transplantation. Compared with the conventional slow freezing method, vitreous cryopreservation has been regarded to be a more promising approach for long-term storage of organs. The major challenges to vitrification are devitrification and recrystallization during the warming process, and high concentrations of cryoprotective agents (CPAs) induced metabolic and osmotic injuries. For a theoretical model based optimization of vitrification, thermal properties of CPA solutions are indispensable. In this study, the thermal conductivities of M22 and vitrification solution containing ethylene glycol and dimethyl sulfoxide (two commonly used vitrification solutions) were measured using a self-made microscaled hot probe with enameled copper wire at the temperature range of 77K-300K. The data obtained by this study will further enrich knowledge of the thermal properties for CPA solutions at low temperatures, as is of primary importance for optimization of vitrification.

引用

页码：228 / 233

页数：6