In Situ Lysosomal Cysteine-Specific Targeting and Imaging during Dexamethasone-Induced Apoptosis

被引：76

作者：

Yue, Yongkang ^{[1
,2
]}

Huo, Fangjun

Yue, Ping ^{[3
]}

Meng, Xiangming ^{[3
]}

Salamanca, James C. ^{[4
]}

Escobedo, Jorge O. ^{[4
]}

Strongin, Robert M. ^{[4
]}

Yin, Caixia ^{[1
,2
]}

机构：

[1] Shanxi Univ, Inst Mol Sci, Key Lab Mat Energy Convers & Storage Shanxi Prov, Minist Educ,Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Shanxi, Peoples R China

[2] Shanxi Univ, Res Inst Appl Chem, Taiyuan 030006, Shanxi, Peoples R China

[3] Anhui Univ, Dept Chem, Hefei 230601, Anhui, Peoples R China

[4] Portland State Univ, Dept Chem, Portland, OR 97201 USA

来源：

ANALYTICAL CHEMISTRY | 2018年 / 90卷 / 11期

基金：

中国国家自然科学基金;

关键词：

RATIOMETRIC FLUORESCENT-PROBE; SELECTIVE DETECTION; ADDITION-REACTIONS; EMISSION CHANNELS; HYDROGEN-SULFIDE; LIVING CELLS; PH CHANGES; LIVE-CELL; GLUTATHIONE; HOMOCYSTEINE;

D O I：

10.1021/acs.analchem.8b01406

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein we utilize the similar though divergent nucleophilic properties of cysteine, homocysteine, and glutathione to achieve the selective detection of cysteine under mildly acidic conditions. This enables the specific in situ detection of lysosomal cysteine. Employing time-dependent fluorescent imaging of probe-labeled AS49 cells, we demonstrate that dexamethasone-induced apoptosis is not dependent on lysosomal cysteine. This methodology can thus produce useful information about pathogenesis associated with cysteine and lysosomes.

引用

页码：7018 / 7024

页数：7

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