Genomic analysis of methicillin-resistant Staphylococcus aureus strain SO-1977 from Sudan

被引：11

作者：

Ali, Mohamed S. ^{[1
]}

Isa, Nurulfiza M. ^{[2
]}

Abedelrhman, Faisal M. ^{[1
]}

Alyas, Tahani B. ^{[3
]}

Mohammed, Sara E. ^{[4
]}

Ahmed, Abdallah E. ^{[1
]}

Ahmed, Zainab S. A. ^{[1
]}

Lau, Nyok-Sean ^{[5
]}

Garbi, Mohamed I. ^{[6
]}

Amirul, Abdullah Al-Ashraf ^{[5
]}

Seed, Almeen O. ^{[1
]}

Omer, Rihab A. ^{[1
]}

Mohamed, Sofia B. ^{[1
]}

机构：

[1] Natl Univ Res Inst Natl Univ, Dept Bioinformat & Biostat, Khartoum, Sudan

[2] Univ Putra Malaysia, Dept Cell & Mol Biol, Fac Biotechnol & Bimol Sci, Serdang, Selangor, Malaysia

[3] Trop Med Res Inst, Khartoum, Sudan

[4] Natl Univ, Fac Med Lab Sci, Dept Microbiol, Khartoum, Sudan

[5] Univ Sains Malaysia, Ctr Chem Biol, Bayan Lepas 11900, Penang, Malaysia

[6] Int Univ Africa, Fac Med Lab Sci, Dept Microbiol, Khartoum, Sudan

来源：

BMC MICROBIOLOGY | 2019年 / 19卷 / 1期

关键词：

Methicillin -resistance Staphylococcus aureus (MRSA); Whole genome sequencing; Antibiotic resistant genes; Genome annotation; Sudan; ANNOTATION; SEQUENCE;

D O I：

10.1186/s12866-019-1470-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnostic assays. The objective of the present study is to perform whole-genome sequencing of MRSA isolated from Sudan using Illumina Next Generation Sequencing (NGS) platform.ResultsThe genome of MRSA strain SO-1977 consists of 2,827,644bp with 32.8% G+C, 59 RNAs and 2629 predicted coding sequences (CDSs). The genome has 26 systems, one of which is the major class in the disease virulence and defence. A total of 83 genes were annotated to virulence disease and defence category some of these genes coding as functional proteins. Based on genome analysis, it is speculated that the SO-1977 strain has resistant genes to Teicoplanin, Fluoroquinolones, Quinolone, Cephamycins, Tetracycline, Acriflavin and Carbapenems. The results revealed that the SO-1977, strain isolated from Sudan has a wide range of antibiotic resistance compared to related strains.ConclusionThe study reports for the first time the whole genome sequence of Sudan MRSA isolates. The release of the genome sequence of the strain SO-1977 will avail MRSA in public databases for further investigations on the evolution of resistant mechanism and dissemination of the -resistant genes of MRSA.

引用

页数：9

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