Genetic engineering of potato for broad-spectrum protection against virus infection

被引：37

作者：

Tacke, E ^{[1
]}

Salamini, F ^{[1
]}

Rohde, W ^{[1
]}

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH,D-50829 COLOGNE,GERMANY

来源：

NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 11期

关键词：

cell-to-cell movement; phloem transport; plasmodesma; Solanum tuberosum;

D O I：

10.1038/nbt1196-1597

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic potato plants expressing mutant alleles of PLRV ORF4, the gene for the movement protein pr17 of this luteovirus, were generated for broad-range protection against virus infection. When tested for protection against infection by PLRV, all transgenic lines showed a significant reduction of virus antigen. Potato lines accumulating N- or C-terminally extended PLRV pr17 mutant proteins were resistant to infection by the unrelated potato viruses PW and PVX. Transgenic lines that did not express protein despite high transcript levels failed to exhibit virus resistance.

引用

页码：1597 / 1601

页数：5

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