CE-LIF coupled with flow cytometry for high-throughput quantitation of fluorophores in single intact cells

被引：11

作者：

Xiao, Hua

Li, Xin

Zou, Hanfa ^{[1
]}

Yang, Ling

Wang, Yulin

Wang, Hailin

Le, X. Chris

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

[2] Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China

[3] Univ Alberta, Fac Med, Dept Publ Hlth Sci, Edmonton, AB, Canada

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 17期

关键词：

apparent permeability coefficient; capillary electrophoresis; flow cytometry; single cell analysis; transport rate;

D O I：

10.1002/elps.200500536

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a method of coupled CE-LIF detection with flow cytometry for high-throughput determination and quantitation of fluorophores in single intact K562/S (KS) cells. The membrane properties of KS cell including fluophore transport rate and apparent permeability coefficient were further quantitatively characterized. The method has advantages for accurate quantitation and unique capacity of high-throughput analysis. The strategy will be useful for the quantitation of fluorophores in the intact cells, such as measurement of multidrug resistance, quantitation of specific protein expression, and quantitative characterization of protein and enzyme functions.

引用

页码：3452 / 3459

页数：8

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