Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

被引：126

作者：

Amit, I

Yakir, L

Katz, M

Zwang, Y

Marmor, MD

Citri, A

Shtiegman, K

Alroy, I

Tuvia, S

Reiss, Y

Roubini, E

Cohen, M

Wides, R

Bacharach, E

Schubert, U

Yarden, Y ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Regulat Biol, IL-76100 Rehovot, Israel

[2] Proteologics Ltd, IL-76124 Rehovot, Israel

[3] Sigma Aldrich Israel Ltd, IL-76100 Rehovot, Israel

[4] Bar Ilan Univ, Dept Life Sci, IL-52900 Ramat Gan, Israel

[5] Tel Aviv Univ, Dept Cell Res & Immunol, IL-69978 Tel Aviv, Israel

[6] Univ Erlangen Nurnberg, Inst Clin & Mol Virol, D-91054 Erlangen, Germany

来源：

GENES & DEVELOPMENT | 2004年 / 18卷 / 14期

关键词：

endocytosis; growth factor; HIV; ubiquitin; signal transduction;

D O I：

10.1101/gad.294904

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

引用

收藏

页码：1737 / 1752

页数：16

相关论文

共 42 条

[11] Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding [J].

Garrus, JE ;

von Schwedler, UK ;

Pornillos, OW ;

Morham, SG ;

Zavitz, KH ;

Wang, HE ;

Wettstein, DA ;

Stray, KM ;

Côté, M ;

Rich, RL ;

Myszka, DG ;

Sundquist, WI .

CELL, 2001, 107 (01) :55-65

[12] Defects in human immunodeficiency virus budding and endosomal sorting induced by TSG101 overexpression [J].

Goila-Gaur, R ;

Demirov, DG ;

Orenstein, JM ;

Ono, A ;

Freed, EO .

JOURNAL OF VIROLOGY, 2003, 77 (11) :6507-6519

[13] Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation [J].

Haglund, K ;

Sigismund, S ;

Polo, S ;

Szymkiewicz, I ;

Di Fiore, PP ;

Dikic, I .

NATURE CELL BIOLOGY, 2003, 5 (05) :461-466

[14] Localization of human immunodeficiency virus type 1 Gag and Env at the plasma membrane by confocal imaging [J].

Hermida-Matsumoto, L ;

Resh, MD .

JOURNAL OF VIROLOGY, 2000, 74 (18) :8670-8679

[15] Regulation of membrane protein transport by ubiquitin and ubiquitin-binding proteins [J].

Hicke, L ;

Dunn, R .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2003, 19 :141-172

[16] Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins [J].

Hittelman, AB ;

Burakov, D ;

Iñiguez-Lluhí, JA ;

Freedman, LP ;

Garabedian, MJ .

EMBO JOURNAL, 1999, 18 (19) :5380-5388

[17] RING finger proteins: Mediators of ubiquitin ligase activity [J].

Joazeiro, CAP ;

Weissman, AM .

CELL, 2000, 102 (05) :549-552

[18] Ligand-independent degradation of epidermal growth factor receptor involves receptor ubiquitylation and hgs, an adaptor whose ubiquitin-interacting motif targets ubiquitylation by Nedd4 [J].

Katz, M ;

Shtiegman, K ;

Tal-Or, P ;

Yakir, L ;

Mosesson, Y ;

Harari, D ;

Machluf, Y ;

Asao, H ;

Jovin, T ;

Sugamura, K ;

Yarden, Y .

TRAFFIC, 2002, 3 (10) :740-751

[19] Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I [J].

Katzmann, DJ ;

Babst, M ;

Emr, SD .

CELL, 2001, 106 (02) :145-155

[20] Vps27 recruits ESCRT machinery to endosomes during MVB sorting [J].

Katzmann, DJ ;

Stefan, CJ ;

Babst, M ;

Emr, SD .

JOURNAL OF CELL BIOLOGY, 2003, 162 (03) :413-423

← 1 2 3 4 5 →