Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding

被引：126

作者：

Amit, I

Yakir, L

Katz, M

Zwang, Y

Marmor, MD

Citri, A

Shtiegman, K

Alroy, I

Tuvia, S

Reiss, Y

Roubini, E

Cohen, M

Wides, R

Bacharach, E

Schubert, U

Yarden, Y ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Regulat Biol, IL-76100 Rehovot, Israel

[2] Proteologics Ltd, IL-76124 Rehovot, Israel

[3] Sigma Aldrich Israel Ltd, IL-76100 Rehovot, Israel

[4] Bar Ilan Univ, Dept Life Sci, IL-52900 Ramat Gan, Israel

[5] Tel Aviv Univ, Dept Cell Res & Immunol, IL-69978 Tel Aviv, Israel

[6] Univ Erlangen Nurnberg, Inst Clin & Mol Virol, D-91054 Erlangen, Germany

来源：

GENES & DEVELOPMENT | 2004年 / 18卷 / 14期

关键词：

endocytosis; growth factor; HIV; ubiquitin; signal transduction;

D O I：

10.1101/gad.294904

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.

引用

页码：1737 / 1752

页数：16

共 42 条

[1] Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking [J].