Isolation and mapping of a C3'H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress

被引：37

作者：

Moglia, Andrea ^{[1
]}

Comino, Cinzia ^{[1
]}

Portis, Ezio ^{[1
]}

Acquadro, Alberto ^{[1
]}

De Vos, Ric C. H. ^{[2
]}

Beekwilder, Jules ^{[2
]}

Lanteri, Sergio ^{[1
]}

机构：

[1] Univ Torino, DiVaPRA Plant Genet & Breeding, I-10095 Grugliasco, TO, Italy

[2] Plant Res Int, NL-6700 AA Wageningen, Netherlands

来源：

PLANT CELL REPORTS | 2009年 / 28卷 / 06期

关键词：

Globe artichoke; C3 ' H; Genetic mapping; UV-C radiation; CHLOROGENIC ACID BIOSYNTHESIS; CULTURED RAT HEPATOCYTES; CYNARA-SCOLYMUS L; PHENYLPROPANOID METABOLISM; FUNCTIONAL-CHARACTERIZATION; ARABIDOPSIS-THALIANA; PHENOLIC-COMPOUNDS; YEAST EXPRESSION; 3'-HYDROXYLASE; 3-HYDROXYLASE;

D O I：

10.1007/s00299-009-0695-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3'-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed maps.

引用

页码：963 / 974

页数：12