Interaction between tubulin and Na+,K+-ATPase in brain stem neurons

被引：6

作者：

Vladimirova, NM ^{[1
]}

Sautkina, EN ^{[1
]}

Ovchinnikova, TV ^{[1
]}

Potapenko, NA ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2002年 / 67卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

Na+; K+-ATPase; isoforms; isozymes; axolemma; tubulin;

D O I：

10.1023/A:1015202610797

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Functionally active Na+,K+-ATPase isozymes containing three types of the catalytic subunits (alpha1, alpha2, and alpha3) were obtained from calf brain by two methods: selective removal of contaminating proteins according to Jorgensen (1974) and selective solubilization of the enzyme with subsequent reformation of the membrane structure according to Esmann (1988). All preparations were characterized with respect to ouabain-inhibition constants. The presence of the cytoskeleton protein tubulin (beta3 isoform) in the high-molecular-weight complex of Na+,K+-ATPase alpha3beta1 isozyme from brain stem axolemma and the junction between Na+,K+-ATPase alpha3 subunit and tubulin beta3 subunit are shown for the first time.

引用

页码：503 / 509

页数：7

共 37 条

[1] CHARACTERIZATION OF POSTTRANSLATIONAL MODIFICATIONS IN NEURON-SPECIFIC CLASS-III BETA-TUBULIN BY MASS-SPECTROMETRY [J].

ALEXANDER, JE ;

HUNT, DF ;

LEE, MK ;

SHABANOWITZ, J ;

MICHEL, H ;

BERLIN, SC ;

MACDONALD, TL ;

SUNDBERG, RJ ;

REBHUN, LI ;

FRANKFURTER, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4685-4689

[2] Isozymes of the Na-K-ATPase: heterogeneity in structure, diversity in function [J].

Blanco, G ;

Mercer, RW .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1998, 275 (05) :F633-F650

[3] Transport and pharmacological properties of nine different human Na,K-ATPase isozymes [J].

Crambert, G ;

Hasler, U ;

Beggah, AT ;

Yu, CL ;

Modyanov, NN ;

Horisberger, JD ;

Lelièvre, L ;

Geering, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (03) :1976-1986

[4] ANKYRIN BINDS TO 2 DISTINCT CYTOPLASMIC DOMAINS OF NA,K-ATPASE ALPHA-SUBUNIT [J].

DEVARAJAN, P ;

SCARAMUZZINO, DA ;

MORROW, JS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :2965-2969

[5] ISOLATION AND PARTIAL CHARACTERIZATION OF RAT CNS AXOLEMMA ENRICHED FRACTIONS [J].

DEVRIES, GH ;

MATTHIEU, JM ;

BENY, M ;

CHICHEPORTICHE, R ;

LAZDUNSKI, M ;

DOLIVO, M .

BRAIN RESEARCH, 1978, 147 (02) :339-352

[6] PRECIPITATION OF SOLUBILIZED NA+ /K+-ATPASE BY DIVALENT-CATIONS [J].

ESMANN, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 940 (01) :71-76

[7] SULFHYDRYL-GROUPS OF (NA+ +K+)-ATPASE FROM RECTAL GLANDS OF SQUALUS-ACANTHIAS - TITRATIONS AND CLASSIFICATION [J].

ESMANN, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 688 (01) :251-259

[8]

FISONE G, 1994, J BIOL CHEM, V269, P9368

[9] THE ADHESION MOLECULE ON GLIA (AMOG) IS A HOMOLOG OF THE BETA-SUBUNIT OF THE NA,K-ATPASE [J].

GLOOR, S ;

ANTONICEK, H ;

SWEADNER, KJ ;

PAGLIUSI, S ;

FRANK, R ;

MOOS, M ;

SCHACHNER, M .

JOURNAL OF CELL BIOLOGY, 1990, 110 (01) :165-174

[10] PURIFICATION AND CHARACTERIZATION OF (NA++K+)-ATPASE .3. PURIFICATION FROM OUTER MEDULLA OF MAMMALIAN KIDNEY AFTER SELECTIVE REMOVAL OF MEMBRANE COMPONENTS BY SODIUM DODECYLSULFATE [J].

JORGENSEN, PL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 356 (01) :36-52

← 1 2 3 4 →